ArticleGenetics2009
Phenotypic consequences of purine nucleotide imbalance in Saccharomyces cerevisiae.
Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies inSynthetic and systems biotechnology · 2025Article
- Phase separation of the PRPP amidotransferase into dynamic condensates promotes de novo purine synthesis in yeast.PLoS biology · 2025Article
- Article
- Purine metabolism in plant pathogenic fungi.Frontiers in microbiology · 2024Review
- Genetic interaction between RLM1 and F-box motif encoding gene SAF1 contributes to stress response in Saccharomyces cerevisiae.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021Article
- Interactions of GMP with Human Glrx3 and with Saccharomyces cerevisiae Grx3 and Grx4 Converge in the Regulation of the Gcn2 Pathway.Applied and environmental microbiology · 2020Article
- Dual control of NADeLife · 2019Article
- Article
- Yeast to Study Human Purine Metabolism Diseases.Cells · 2019Review
- Article
- Disruption of Nucleotide Homeostasis by the Antiproliferative Drug 5-Aminoimidazole-4-carboxamide-1-β-d-ribofuranoside Monophosphate (AICAR).The Journal of biological chemistry · 2015Article
- A mutation in Ampd2 is associated with nephrotic syndrome and hypercholesterolemia in mice.Lipids in health and disease · 2014Article
- Article
- Developmental stage dependent metabolic regulation during meiotic differentiation in budding yeast.BMC biology · 2014Article
- Identification of yeast and human 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAr) transporters.The Journal of biological chemistry · 2014Article
- Purification and partial characterization of NAD aminohydrolase from Aspergillus oryzae NRRL447.World journal of microbiology & biotechnology · 2014Article
- Article
- Article
- Tye7 regulates yeast Ty1 retrotransposon sense and antisense transcription in response to adenylic nucleotides stress.Nucleic acids research · 2012Article
- Dynamic metabolomics differentiates between carbon and energy starvation in recombinant Saccharomyces cerevisiae fermenting xylose.Biotechnology for biofuels · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coordinating homeostasis of multiple metabolites is a major task for living organisms, and complex interconversion pathways contribute to achieving the proper balance of metabolites. AMP deaminase (AMPD) is such an interconversion enzyme that allows IMP synthesis from AMP. In this article, we show that, under specific conditions, lack of AMPD activity impairs growth. Under these conditions, we found that the intracellular guanylic nucleotide pool was severely affected. In vivo studies of two AMPD homologs, Yjl070p and Ybr284p, indicate that these proteins have no detectable AMP, adenosine, or adenine deaminase activity; we show that overexpression of YJL070c instead mimics a loss of AMPD function. Expression of the yeast transcriptome was monitored in a AMPD-deficient mutant in a strain overexpressing YJL070c and in cells treated with the immunosuppressive drug mycophenolic acid, three conditions that lead to severe depletion of the guanylic nucleotide pool. These three conditions resulted in the up- or downregulation of multiple transcripts, 244 of which are common to at least two conditions and 71 to all three conditions. These transcriptome results, combined with specific mutant analysis, point to threonine metabolism as exquisitely sensitive to the purine nucleotide balance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.