ArticleMolecular and cellular biology2009
Assembly of an export-competent mRNP is needed for efficient release of the 3'-end processing complex after polyadenylation.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 66 citations in OpenAlex.
- DEAD-box ATPase Dbp2 is the key enzyme in an mRNP assembly checkpoint at the 3'-end of genes and involved in the recycling of cleavage factors.Nature communications · 2024Article
- Article
- Birth of a poly(A) tail: mechanisms and control of mRNA polyadenylation.FEBS open bio · 2023Review
- Nuclear mRNA Export and Aging.International journal of molecular sciences · 2022Review
- Three-layered control of mRNA poly(A) tail synthesis inGenes & development · 2021Article
- Inhibition of Host Gene Expression by KSHV: Sabotaging mRNA Stability and Nuclear Export.Frontiers in cellular and infection microbiology · 2021Review
- Telomerase biogenesis requires a novel Mex67 function and a cytoplasmic association with the SmeLife · 2020Article
- Strength in Diversity: Nuclear Export of Viral RNAs.Viruses · 2020Review
- Co-transcriptional Loading of RNA Export Factors Shapes the Human Transcriptome.Molecular cell · 2019Article
- Genome-Wide Discovery of DEAD-Box RNA Helicase Targets Reveals RNA Structural Remodeling in Transcription Termination.Genetics · 2019Article
- Polyadenylation and nuclear export of mRNAs.The Journal of biological chemistry · 2019Review
- Chimeric 3' flanking regions strongly enhance gene expression in plants.Plant biotechnology journal · 2018Article
- An intronless form of the tobacco extensin gene terminator strongly enhances transient gene expression in plant leaves.Plant molecular biology · 2018Article
- Single-Cell and Single-Molecule Analysis of Gene Expression Regulation.Annual review of genetics · 2016Review
- The role of TREX in gene expression and disease.The Biochemical journal · 2016Review
- Defects in THO/TREX-2 function cause accumulation of novel cytoplasmic mRNP granules that can be cleared by autophagy.RNA (New York, N.Y.) · 2016Article
- Deciphering the Structure and Function of Nuclear Pores Using Single-Molecule Fluorescence Approaches.Journal of molecular biology · 2016Review
- Macromolecular transport between the nucleus and the cytoplasm: Advances in mechanism and emerging links to disease.Biochimica et biophysica acta · 2014Review
- The DEAD-box protein Dbp2 functions with the RNA-binding protein Yra1 to promote mRNP assembly.Journal of molecular biology · 2013Article
- mRNA quality control pathways in Saccharomyces cerevisiae.Journal of biosciences · 2013Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Before polyadenylated mRNA is exported from the nucleus, the 3'-end processing complex is removed by a poorly described mechanism. In this study, we asked whether factors involved in mRNP maturation and export are also required for disassembly of the cleavage and polyadenylation complex. An RNA immunoprecipitation assay monitoring the amount of the cleavage factor (CF) IA component Rna15p associated with poly(A)(+) RNA reveals defective removal of Rna15p in mutants of the nuclear export receptor Mex67p as well as other factors important for assembly of an export-competent mRNP. In contrast, Rna15p is not retained in mutants of export factors that function primarily on the cytoplasmic side of the nuclear pore. Consistent with a functional interaction between Mex67p and the 3'-end processing complex, a mex67 mutant accumulates unprocessed SSA4 transcripts and exhibits a severe growth defect when this mutation is combined with mutation of Rna15p or another CF IA subunit, Rna14p. RNAs that become processed in a mex67 mutant have longer poly(A) tails both in vivo and in vitro. This influence of Mex67p on 3'-end processing is conserved, as depletion of its human homolog, TAP/NXF1, triggers mRNA hyperadenylation. Our results indicate a function for nuclear mRNP assembly factors in releasing the 3'-end processing complex once polyadenylation is complete.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.