ArticleThe Journal of biological chemistry2009
Unraveling delta1-pyrroline-5-carboxylate-proline cycle in plants by uncoupled expression of proline oxidation enzymes.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
92 citing papers in PubMed, 252 citations in OpenAlex.
- Evolutionary and Structural Insights into Proline Metabolism Genes Associated with Salt Resilience in Mango.Biochemical genetics · 2026Article
- Evolutionary characteristics of the GAPDH gene family and functional characterization of TaGAPDH5 in wheat.BMC plant biology · 2026Article
- Bioformulations of Entrophospora lutea enriched with biostimulants for growth promotion and control of Rhizoctonia root rot in lupine.Scientific reports · 2026Article
- Elevated COMycorrhiza · 2026Article
- The Substrate Versatility of ΔMolecules (Basel, Switzerland) · 2026Article
- Quantification of Mitochondrial Pyrroline-5-Carboxylate in Arabidopsis Under Pseudomonas Syringae Infection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Plant Biotic Stress: Tools and Techniques for Crop Protection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Measurement of δMetabolites · 2025Article
- Proline transporters balance the salicylic acid-mediated trade-off between regeneration and immunity in plants.bioRxiv : the preprint server for biology · 2025Article
- The Proline Dehydrogenase GeneInternational journal of molecular sciences · 2025Article
- Proline and ROS: A Unified Mechanism in Plant Development and Stress Response?Plants (Basel, Switzerland) · 2024Review
- The nonphototrophic hypocotyl 3 (NPH3) domain protein NRL5 is a trafficking-associated GTPase essential for drought resistance.Science advances · 2024Article
- Plants interfere with non-self recognition of a phytopathogenic fungus via proline accumulation to facilitate mycovirus transmission.Nature communications · 2024Article
- Overexpression and knockdown of cottoniScience · 2024Article
- Proline-mediated redox regulation in wheat for mitigating nickel-induced stress and soil decontamination.Scientific reports · 2024Article
- Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress.The Plant cell · 2023Article
- Article
- Interplay between Proline Metabolism and ROS in the Fine Tuning of Root-Meristem Size inPlants (Basel, Switzerland) · 2022Article
- HAntioxidants (Basel, Switzerland) · 2022Article
- Alternative oxidase (AOX) 1a and 1d limit proline-induced oxidative stress and aid salinity recovery in Arabidopsis.Plant physiology · 2022Article
32 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The two-step oxidation of proline in all eukaryotes is performed at the inner mitochondrial membrane by the consecutive action of proline dehydrogenase (ProDH) that produces Delta(1)-pyrroline-5-carboxylate (P5C) and P5C dehydrogenase (P5CDH) that oxidizes P5C to glutamate. This catabolic route is down-regulated in plants during osmotic stress, allowing free Pro accumulation. We show here that overexpression of MsProDH in tobacco and Arabidopsis or impairment of P5C oxidation in the Arabidopsis p5cdh mutant did not change the cellular Pro to P5C ratio under ambient and osmotic stress conditions, indicating that P5C excess was reduced to Pro in a mitochondrial-cytosolic cycle. This cycle, involving ProDH and P5C reductase, exists in animal cells and now demonstrated in plants. As a part of the cycle, Pro oxidation by the ProDH-FAD complex delivers electrons to the electron transport chain. Hyperactivity of the cycle, e.g. when an excess of exogenous l-Pro is provided, generates mitochondrial reactive oxygen species (ROS) by delivering electrons to O(2), as demonstrated by the mitochondria-specific MitoSox staining of superoxide ions. Lack of P5CDH activity led to higher ROS production under dark and light conditions in the presence of Pro excess, as well as rendered plants hypersensitive to heat stress. Balancing mitochondrial ROS production during increased Pro oxidation is therefore critical for avoiding Pro-related toxic effects. Hence, normal oxidation of P5C to Glu by P5CDH is key to prevent P5C-Pro intensive cycling and avoid ROS production from electron run-off.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.