ArticleAnnals of botany2009
Comparative cytogenetic analysis of the genomes of the model grass Brachypodium distachyon and its close relatives.
Article in Annals of botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 64 citations in OpenAlex.
- Expansions and contractions of repetitive DNA elements reveal contrasting evolutionary responses to the polyploid genome shock hypothesis inFrontiers in plant science · 2024Article
- Article
- Genomic constitution, allopolyploidy, and evolutionary proposal for Cynodon Rich. based on GISH.Protoplasma · 2022Article
- Insight into the Chromosome Structure of the Cultivated Tetraploid Alfalfa (Plants (Basel, Switzerland) · 2020Article
- In Vitro Tissue Culture inInternational journal of molecular sciences · 2020Review
- Chromosome Painting Based on Bulked Oligonucleotides in Cotton.Frontiers in plant science · 2020Article
- Comparative molecular cytogenetic characterization of five wildComparative cytogenetics · 2020Article
- Comparatively Barcoded Chromosomes ofInternational journal of molecular sciences · 2019Article
- Molecular cytogenetic characterization and phylogenetic analysis of fourComparative cytogenetics · 2019Article
- FISH-aimed karyotype analysis in Aconitum subgen. Aconitum reveals excessive rDNA sites in tetraploid taxa.Protoplasma · 2018Article
- Chromosome identification and reconstruction of evolutionary rearrangements in Brachypodium distachyon, B. stacei and B. hybridum.Annals of botany · 2018Article
- Review
- Neutral molecular markers support common origin of aluminium tolerance in three congeneric grass species growing in acidic soils.AoB PLANTS · 2017Article
- Brachypodium distachyon - A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH.PloS one · 2017Article
- Molecular cytogenetic characterization and comparison of the two cultivatedComparative cytogenetics · 2017Article
- Recreating Stable Brachypodium hybridum Allotetraploids by Uniting the Divergent Genomes of B. distachyon and B. stacei.PloS one · 2016Article
- Karyotype characterization and evolution in South American species of Lathyrus (Notolathyrus, Leguminosae) evidenced by heterochromatin and rDNA mapping.Journal of plant research · 2015Article
- Spatial distribution of centromeres and telomeres at interphase varies among Brachypodium species.Journal of experimental botany · 2015Article
- Molecular characterisation and evolution of HMW glutenin subunit genes in Brachypodium distachyon L.Journal of applied genetics · 2014Article
- Reconstructing the Evolution of Brachypodium Genomes Using Comparative Chromosome Painting.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsBrachypodium is a small genus of temperate grasses that comprises 12-15 species. Brachypodium distachyon is now well established as a model species for temperate cereals and forage grasses. In contrast to B. distachyon, other members of the genus have been poorly investigated at the chromosome level or not at all.
methodsTwenty accessions comprising six species and two subspecies of Brachypodium were analysed cytogenetically. Measurements of nuclear genome size were made by flow cytometry. Chromosomal localization of 18-5.8-25S rDNA and 5S rDNA loci was performed by dual-colour fluorescence in situ hybridization (FISH) on enzymatically digested root-tip meristematic cells. For comparative phylogenetic analyses genomic in situ hybridization (GISH) applied to somatic chromosome preparations was used. KEY
resultsAll Brachypodium species examined have rather small genomes and chromosomes. Their chromosome numbers and genome sizes vary from 2n = 10 and 0.631 pg/2C in B. distachyon to 2n = 38 and 2.57 pg/2C in B. retusum, respectively. Genotypes with 18 and 28 chromosomes were found among B. pinnatum accessions. GISH analysis revealed that B. pinnatum with 28 chromosomes is most likely an interspecific hybrid between B. distachyon (2n = 10) and B. pinnatum (2n = 18). Two other species, B. phoenicoides and B. retusum, are also allopolyploids and B. distachyon or a close relative seems to be one of their putative ancestral species. In chromosomes of all species examined the 45S rDNA loci are distally distributed whereas loci for 5S rDNA are pericentromeric.
conclusionsThe increasing significance of B. distachyon as a model grass emphasizes the need to understand the evolutionary relationships in the genus Brachypodium and to ensure consistency in the biological nomenclature of its species. Modern molecular cytogenetic techniques such as FISH and GISH are suitable for comparative phylogenetic analyses and may provide informative chromosome- and/or genome-specific landmarks.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.