ArticleAnnals of botany2009
Mesophyll distribution of 'antioxidant' flavonoid glycosides in Ligustrum vulgare leaves under contrasting sunlight irradiance.
Article in Annals of botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed, 187 citations in OpenAlex.
- Insights into stress responsiveness of MusaANS promoter- an anthocyanidin synthase gene from banana.Protoplasma · 2026Article
- A Two-Stage Screening-to-Optimization Approach with Mechanistic Model Analysis: Enhancing Anthocyanin in Lettuce Without Yield Loss.Plants (Basel, Switzerland) · 2026Article
- Solvent Fractionation ofInternational journal of molecular sciences · 2025Article
- The transcriptome landscape of Kumrogarh, a unique rice landrace showing the simultaneous presence of3 Biotech · 2025Article
- The Impact of Temperature on the Leaves ofPlants (Basel, Switzerland) · 2025Article
- Effect of the Aqueous Quercetin Solution on the Physiological Properties of Virginia Mallow (International journal of molecular sciences · 2025Article
- Antioxidants by nature: an ancient feature at the heart of flavonoids' multifunctionality.The New phytologist · 2025Article
- Exploring the structural variability in developing wheat grains using autofluorescence multispectral imaging at the macroscopic scale.Frontiers in plant science · 2025Article
- Flavonoids and Other Phenolic Compounds for Physiological Roles, Plant Species Delimitation, and Medical Benefits: A Promising View.Molecules (Basel, Switzerland) · 2024Review
- Screening and validation of reference genes in Dracaena cochinchinensis using quantitative real-time PCR.Scientific reports · 2024Article
- Comparative transcriptome analysis reveals the potential mechanism of GAFrontiers in plant science · 2024Article
- Integrated metabolome and transcriptome analysis provide insight into the biosynthesis of flavonoids inFrontiers in plant science · 2024Article
- Rosmarinic Acid and Flavonoids of the SeagrassPlants (Basel, Switzerland) · 2023Article
- How do barley plants with impaired photosynthetic light acclimation survive under high-light stress?Planta · 2023Article
- Leaf Functional Traits in Relation to Species Composition in an Arctic-Alpine Tundra Grassland.Plants (Basel, Switzerland) · 2023Article
- Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions.International journal of molecular sciences · 2023Article
- Diverse Physiological Roles of Flavonoids in Plant Environmental Stress Responses and Tolerance.Plants (Basel, Switzerland) · 2022Review
- A chromosome-level genome assembly for Dracaena cochinchinensis reveals the molecular basis of its longevity and formation of dragon's blood.Plant communications · 2022Article
- Bioactivity and Therapeutic Potential of Kaempferol and Quercetin: New Insights for Plant and Human Health.Plants (Basel, Switzerland) · 2022Review
- Beyond Photoprotection: The Multifarious Roles of Flavonoids in Plant Terrestrialization.International journal of molecular sciences · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsFlavonoids have the potential to serve as antioxidants in addition to their function of UV screening in photoprotective mechanisms. However, flavonoids have long been reported to accumulate mostly in epidermal cells and surface organs in response to high sunlight. Therefore, how leaf flavonoids actually carry out their antioxidant functions is still a matter of debate. Here, the distribution of flavonoids with effective antioxidant properties, i.e. the orthodihydroxy B-ring-substituted quercetin and luteolin glycosides, was investigated in the mesophyll of Ligustrum vulgare leaves acclimated to contrasting sunlight irradiance.
methodsIn the first experiment, plants were grown at 20 % (shade) or 100% (sun) natural sunlight. Plants were exposed to 100 % sunlight irradiance in the presence or absence of UV wavelengths, in a second experiment. Fluorescence microspectroscopy and multispectral fluorescence microimaging were used in both cross sections and intact leaf pieces to visualize orthodihydroxy B-ring-substituted flavonoids at inter- and intracellular levels. Identification and quantification of individual hydroxycinnamates and flavonoid glycosides were performed via HPLC-DAD. KEY
resultsQuercetin and luteolin derivatives accumulated to a great extent in both the epidermal and mesophyll cells in response to high sunlight. Tissue fluorescence signatures and leaf flavonoid concentrations were strongly related. Monohydroxyflavone glycosides, namely luteolin 4'-O-glucoside and two apigenin 7-O-glycosides were unresponsive to changes in sunlight irradiance. Quercetin and luteolin derivatives accumulated in the vacuoles of mesophyll cells in leaves growing under 100 % natural sunlight in the absence of UV wavelengths.
conclusionsThe above findings lead to the hypothesis that flavonoids play a key role in countering light-induced oxidative stress, and not only in avoiding the penetration of short solar wavelengths in the leaf.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.