ArticleApplied and environmental microbiology2009
rhs genes are potential markers for multilocus sequence typing of Escherichia coli O157:H7 strains.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Genetic and Functional Analyses of Virulence Potential of anFrontiers in cellular and infection microbiology · 2020Article
- First step in using molecular data for microbial food safety risk assessment; hazard identification of Escherichia coli O157:H7 by coupling genomic data with in vitro adherence to human epithelial cells.International journal of food microbiology · 2015Article
- Multilocus genotypic characterization of Escherichia coli O157:H7 recovered from food sources.Epidemiology and infection · 2015Article
- Phylogenetic Clades 6 and 8 of Enterohemorrhagic Escherichia coli O157:H7 With Particular stx Subtypes are More Frequently Found in Isolates From Hemolytic Uremic Syndrome Patients Than From Asymptomatic Carriers.Open forum infectious diseases · 2014Article
- Rhs proteins from diverse bacteria mediate intercellular competition.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Phylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative Shiga toxin 1 and 2 prophages.Applied and environmental microbiology · 2012Article
- Differentiation of Xylella fastidiosa strains via multilocus sequence analysis of environmentally mediated genes (MLSA-E).Applied and environmental microbiology · 2012Article
- Genetic features differentiating bovine, food, and human isolates of shiga toxin-producing Escherichia coli O157 in The Netherlands.Journal of clinical microbiology · 2012Article
- Analysis of Escherichia coli O157 clinical isolates by multilocus sequence typing.BMC research notes · 2010Article
- Identification of phylogenetic position in the Chlamydiaceae family for Chlamydia strains released from monkeys and humans with chlamydial pathology.Infectious diseases in obstetrics and gynecology · 2010Article
- Evolutionary diversification of an ancient gene family (rhs) through C-terminal displacement.BMC genomics · 2009Article
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3 authors.
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Abstract
DNA sequence-based molecular subtyping methods such as multilocus sequence typing (MLST) are commonly used to generate phylogenetic inferences for monomorphic pathogens. The development of an effective MLST scheme for subtyping Escherichia coli O157:H7 has been hindered in the past due to the lack of sequence variation found within analyzed housekeeping and virulence genes. A recent study suggested that rhs genes are under strong positive selection pressure, and therefore in this study we analyzed these genes within a diverse collection of E. coli O157:H7 strains for sequence variability. Eighteen O157:H7 strains from lineages I and II and 15 O157:H7 strains from eight clades were included. Examination of these rhs genes revealed 44 polymorphic loci (PL) and 10 sequence types (STs) among the 18 lineage strains and 280 PL and 12 STs among the 15 clade strains. Phylogenetic analysis using rhs genes generally grouped strains according to their known lineage and clade classifications. These findings also suggested that O157:H7 strains from clades 6 and 8 fall into lineage I/II and that strains of clades 1, 2, 3, and 4 fall into lineage I. Additionally, unique markers were found in rhsA and rhsJ that might be used to define clade 8 and clade 6. Therefore, rhs genes may be useful markers for phylogenetic analysis of E. coli O157:H7.
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