ArticleBiochimica et biophysica acta2009
PKCdelta influences p190 phosphorylation and activity: events independent of PKCdelta-mediated regulation of endothelial cell stress fiber and focal adhesion formation and barrier function.
Article in Biochimica et biophysica acta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Rho GTPase-activating protein 35 rs1052667 polymorphism and osteosarcoma risk and prognosis.BioMed research international · 2014Trial
- Regulation of Rho GTPase activity at the leading edge of migrating cells by p190RhoGAP.Small GTPases · 2019Review
- Glucocorticoid receptor DNA binding factor 1 expression and osteosarcoma prognosis.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- Endothelin-1 stimulates catalase activity through the PKCδ-mediated phosphorylation of serine 167.Free radical biology & medicine · 2014Article
- PKCδ regulates force signaling during VEGF/CXCL4 induced dissociation of endothelial tubes.PloS one · 2014Article
- Interplay between FAK, PKCδ, and p190RhoGAP in the regulation of endothelial barrier function.Microvascular research · 2012Review
- Cigarette smoke causes lung vascular barrier dysfunction via oxidative stress-mediated inhibition of RhoA and focal adhesion kinase.American journal of physiology. Lung cellular and molecular physiology · 2011Article
- Inhibition of the AMP-activated protein kinase-α2 accentuates agonist-induced vascular smooth muscle contraction and high blood pressure in mice.Hypertension (Dallas, Tex. : 1979) · 2011Article
- Role of protein tyrosine phosphatase SHP2 in barrier function of pulmonary endothelium.American journal of physiology. Lung cellular and molecular physiology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundWe have shown that protein kinase Cdelta (PKCdelta) inhibition results in increased endothelial cell (EC) permeability and decreased RhoA activity; which correlated with diminished stress fibers (SF) and focal adhesions (FA). We have also shown co-precipitation of p190RhoGAP (p190) with PKCdelta. Here, we investigated if PKCdelta regulates p190 and whether PKCdelta-mediated changes in SF and FA or permeability were dependent upon p190.
methodsProtein-protein interaction and activity analyses were performed using co-precipitation assays. Analysis of p190 phosphorylation was performed using in vitro kinase assays. SF and FA were analyzed by immunofluorescence analyses. EC monolayer permeability was measured using electrical cell impedance sensor (ECIS) technique.
resultsInhibition of PKCdelta increased p190 activity, while PKCdelta overexpression diminished p190 activity. PKCdelta bound to and phosphorylated both p190FF and p190GTPase domains. p190 protein overexpression diminished SF and FA formation and RhoA activity. Disruption of SF and FA or increased permeability induced upon PKCdelta inhibition, were not attenuated in EC in which the p190 isoforms were suppressed individually or concurrently. GENERAL SIGNIFICANCE: Our findings suggest that while PKCdelta can regulate p190 activity, possibly at the FF and/or GTPase domains, the effect of PKCdelta inhibition on SF and FA and barrier dysfunction occurs through a pathway independent of p190.
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