ArticleJournal of molecular and cellular cardiology2010
Differential effects of the transient outward K(+) current activator NS5806 in the canine left ventricle.
Article in Journal of molecular and cellular cardiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- KCells · 2023Review
- Inducing IJournal of molecular and cellular cardiology · 2022Article
- Pharmacological Approaches for the Modulation of the Potassium Channel KInternational journal of molecular sciences · 2021Review
- Auxiliary subunits control biophysical properties and response to compound NS5806 of the Kv4 potassium channel complex.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Interventricular differences in sodium current and its potential role in Brugada syndrome.Physiological reports · 2018Article
- Regulation of Kv4.3 and hERG potassium channels by KChIP2 isoforms and DPP6 and response to the dual KBiochemical pharmacology · 2018Article
- Biophysical and molecular comparison of sodium current in cells isolated from canine atria and pulmonary vein.Pflugers Archiv : European journal of physiology · 2017Article
- Differential responses of rabbit ventricular and atrial transient outward current (IPhysiological reports · 2017Article
- Transmural gradients in ion channel and auxiliary subunit expression.Progress in biophysics and molecular biology · 2016Review
- Intact Heart Loose Patch Photolysis Reveals Ionic Current Kinetics During Ventricular Action Potentials.Circulation research · 2016Article
- Myocardial KChIP2 Expression in Guinea Pig Resolves an Expanded Electrophysiologic Role.PloS one · 2016Article
- Cardiovascular Action of Insulin in Health and Disease: Endothelial L-Arginine Transport and Cardiac Voltage-Dependent Potassium Channels.Frontiers in physiology · 2016Review
- Regional variation of the inwardly rectifying potassium current in the canine heart and the contributions to differences in action potential repolarization.Journal of molecular and cellular cardiology · 2015Article
- Transient outward potassium channel: a heart failure mediator.Heart failure reviews · 2015Review
- Modulation of the voltage-gated potassium channel (Kv4.3) and the auxiliary protein (KChIP3) interactions by the current activator NS5806.The Journal of biological chemistry · 2014Article
- Developmental changes in expression and biophysics of ion channels in the canine ventricle.Journal of molecular and cellular cardiology · 2013Article
- Identification and characterization of a transient outward K+ current in human induced pluripotent stem cell-derived cardiomyocytes.Journal of molecular and cellular cardiology · 2013Article
- Physiological consequences of transient outward K+ current activation during heart failure in the canine left ventricle.Journal of molecular and cellular cardiology · 2012Article
- Impact of KChIP2 on Cardiac Electrophysiology and the Progression of Heart Failure.Frontiers in physiology · 2012Article
- Comparison of the effects of a transient outward potassium channel activator on currents recorded from atrial and ventricular cardiomyocytes.Journal of cardiovascular electrophysiology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
To examine the electrophysiological and molecular properties of the transient outward current (I(to)) in canine left ventricle using a novel I(to) activator, NS5806, I(to) was measured in isolated epicardial (Epi), midmyocardial (Mid) and endocardial (Endo) cells using whole-cell patch-clamp techniques. NS5806 activation of K(v)4.3 current was also studied in CHO-K1 cells and Xenopus laevis oocytes. In CHO-K1 cells co-transfected with K(v)4.3 and KChIP2, NS5806 (10 microM) caused a 35% increase in current amplitude and a marked slowing of current decay with tau increasing from 7.0+/-0.4 to 10.2+/-0.3 ms. In the absence of KChIP2, current decay was unaffected by NS5806. In ventricular myocytes, NS5806 increased I(to) density by 80%, 82%, and 16% in Epi, Mid, and Endo myocytes, respectively (at +40 mV) and shifted steady-state inactivation to negative potentials. NS5806 also significantly slowed decay of I(to), increasing total charge to 227%, 192% and 83% of control in Epi, Mid and Endo cells, respectively (+40 mV, p<0.05). Quantification of K(v)4.3 and KChIP2 mRNA in the 3 ventricular cell types revealed that levels of K(v)4.3 message was uniform but those of KChIP2 were significantly greater in Epi and Mid cells. The KChIP2 gradient was confirmed at the protein level by Western blot. Our results suggest that NS5806 augments I(to) by increasing current density and slowing decay and that both depend on the presence of KChIP2. I(to) and its augmentation by NS5806 are greatest in Epi and Mid cells because KChIP2 levels are highest in these cell types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.