ArticleThe Histochemical journal1990
Discrimination of closely homologous HPV types by nonisotopic in situ hybridization: definition and derivation of tissue melting temperatures.
Article in The Histochemical journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Comparative analysis of human papillomavirus detection by PCR and non-isotopic in situ hybridisation.Journal of clinical pathology · 1995Trial
- Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens.BMC clinical pathology · 2003Article
- In cell amplification.Molecular pathology : MP · 1998Review
- Discrimination of closely homologous human genomic and viral sequences in cells and tissues: further characterization of Tmt.The Histochemical journal · 1994Article
- The discrimination of high-risk HPV types by in situ hybridization and the polymerase chain reaction.The Histochemical journal · 1993Article
- Differential expression of four genes encoding molluscan insulin-related peptides in the central nervous system of the pond snail Lymnaea stagnalis.Cell and tissue research · 1992Article
- Detection of high risk human papillomavirus in routine cervical smears: strategy for screening.Journal of clinical pathology · 1992Article
- Laboratory techniques in the investigation of human papillomavirus infection.Genitourinary medicine · 1992Review
- Interphase cytogenetics using biotin and digoxigenin labelled probes: III. Increased sensitivity and flexibility for detecting HPV in cervical biopsy specimens and cell lines.Journal of clinical pathology · 1991Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is generally assumed that nucleic acid association during in situ hybridization reactions is similar to that of nucleic acid association in solution. This assumption has been investigated by detecting closely homologous human papillomavirus types 6 and 11 by in situ hybridization as a model for the evaluation of stringency conditions in clinical biopsies. By examining matched and mismatched, labelled and target sequences under various stringency conditions, empirical DNA-DNA stability curves and their derivative equations for tissue melting temperatures (Tmt) were derived. The corresponding values for Tmt are 10-20 degrees C higher than their solution equivalents. These data, supported by polymerase chain reaction experiments, demonstrate that closely homologous viral DNAs cross linked in tissue by formaldehyde fixation do not interact with the corresponding labelled probes as predicted from solution kinetic equations. This not only has theoretical implications but is also relevant to the accuracy of clinical diagnostic testing.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.