ArticleVirology2009
Interaction of HCMV UL84 with C/EBPalpha transcription factor binding sites within oriLyt is essential for lytic DNA replication.
Article in Virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cells.PLoS pathogens · 2013Trial
- Effect of host telomerase inhibition on human cytomegalovirus.Journal of virology · 2025Article
- G-quadruplex as an essential structural element in cytomegalovirus replication origin.Nature communications · 2024Article
- Review
- Characteristics of Immediate-Early 2 (IE2) and UL84 Proteins in UL84-Independent Strains of Human Cytomegalovirus (HCMV).Microbiology spectrum · 2021Article
- Human cytomegalovirus long noncoding RNA4.9 regulates viral DNA replication.PLoS pathogens · 2020Article
- Human Cytomegalovirus Primary Infection and Reactivation: Insights From Virion-Carried Molecules.Frontiers in microbiology · 2020Review
- pUL34 binding near the human cytomegalovirus origin of lytic replication enhances DNA replication and viral growth.Virology · 2018Article
- Dynamic and nucleolin-dependent localization of human cytomegalovirus UL84 to the periphery of viral replication compartments and nucleoli.Journal of virology · 2014Article
- Functional annotation of human cytomegalovirus gene products: an update.Frontiers in microbiology · 2014Review
- Analysis of the interactions of viral and cellular factors with human cytomegalovirus lytic origin of replication, oriLyt.Virology · 2012Article
- Depletion of cellular pre-replication complex factors results in increased human cytomegalovirus DNA replication.PloS one · 2012Article
- Interaction of Kaposi's sarcoma-associated herpesvirus ORF59 with oriLyt is dependent on binding with K-Rta.Journal of virology · 2011Article
- Nucleocytoplasmic shuttling of human cytomegalovirus UL84 is essential for virus growth.Journal of virology · 2010Article
- Initiation of lytic DNA replication in Epstein-Barr virus: search for a common family mechanism.Future virology · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Human cytomegalovirus (HCMV) lytic DNA replication is initiated at the cis-acting oriLyt region and requires six core replication proteins along with UL84 and IE2. Although UL84 is thought to be the replication initiator protein, little is known about its interaction with oriLyt. We have now performed chromatin immunoprecipitation assays (ChIP) using antibodies specific to UL84, IE2, UL44, CCAAT/enhancer binding protein (C/EBPalpha) and PCR primers that span the entire oriLyt region to reveal an evaluation of specific protein binding across oriLyt. UL84 interacted with several regions of oriLyt that contain C/EBPalpha transcription factor binding sites. Mutation of either of one of C/EBPalpha (92,526 or 92,535) sites inactivated oriLyt and resulted in the loss of binding of UL84. These data reveal the regions of interaction within oriLyt for several key replication proteins and show that the interaction between UL84 and C/EBPalpha sites within oriLyt is essential for lytic DNA replication.
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