ArticleToxicology and applied pharmacology2009
Nanoimages show disruption of tubulin polymerization by chlorpyrifos oxon: implications for neurotoxicity.
Article in Toxicology and applied pharmacology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Risk Factors for Brain Health in Agricultural Work: A Systematic Review.International journal of environmental research and public health · 2022Pooled it
- Mechanisms of Neurotoxicity of Organophosphate Pesticides and Their Relation to Neurological Disorders.Neuropsychiatric disease and treatment · 2024Review
- Is Gulf War Illness a prolonged early phase tauopathy?Cytoskeleton (Hoboken, N.J.) · 2024Article
- Retinal Toxicity Induced by Chemical Agents.International journal of molecular sciences · 2022Review
- Prenatal exposure to organophosphate pesticides and brain morphology and white matter microstructure in preadolescents.Environmental research · 2020Article
- Nile Tilapia Derived Antimicrobial Peptide TP4 Exerts Antineoplastic Activity Through Microtubule Disruption.Marine drugs · 2018Article
- Transcriptome analysis on chlorpyrifos detoxification in Uronema marinum (Ciliophora, Oligohymenophorea).Environmental science and pollution research international · 2018Article
- Chlorpyrifos and chlorpyrifos oxon impair the transport of membrane bound organelles in rat cortical axons.Neurotoxicology · 2017Article
- Article
- AnToxicology research · 2016Article
- Diisopropylfluorophosphate Impairs the Transport of Membrane-Bound Organelles in Rat Cortical Axons.The Journal of pharmacology and experimental therapeutics · 2016Article
- Neurotoxicity in Preclinical Models of Occupational Exposure to Organophosphorus Compounds.Frontiers in neuroscience · 2016Review
- Prenatal exposure of guinea pigs to the organophosphorus pesticide chlorpyrifos disrupts the structural and functional integrity of the brain.Neurotoxicology · 2015Article
- Prenatal organophosphates exposure alternates the cleavage plane orientation of apical neural progenitor in developing neocortex.PloS one · 2014Article
- Increased gut permeability and bacterial translocation after chronic chlorpyrifos exposure in rats.PloS one · 2014Article
- Cresyl saligenin phosphate, an organophosphorus toxicant, makes covalent adducts with histidine, lysine, and tyrosine residues of human serum albumin.Chemical research in toxicology · 2012Article
- Functional consequences of repeated organophosphate exposure: potential non-cholinergic mechanisms.Pharmacology & therapeutics · 2012Review
- Subacute developmental exposure of zebrafish to the organophosphate pesticide metabolite, chlorpyrifos-oxon, results in defects in Rohon-Beard sensory neuron development.Aquatic toxicology (Amsterdam, Netherlands) · 2010Article
- Review of tyrosine and lysine as new motifs for organophosphate binding to proteins that have no active site serine.Chemico-biological interactions · 2010Review
- Detection of adduct on tyrosine 411 of albumin in humans poisoned by dichlorvos.Toxicological sciences : an official journal of the Society of Toxicology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Organophosphorus agents cause cognitive deficits and depression in some people. We hypothesize that the mechanism by which organophosphorus agents cause these disorders is by modification of proteins in the brain. One such protein could be tubulin. Tubulin polymerizes to make the microtubules that transport cell components to nerve axons. The goal of the present work was to measure the effect of the organophosphorus agent chlorpyrifos oxon on tubulin polymerization. An additional goal was to identify the amino acids covalently modified by chlorpyrifos oxon in microtubule polymers and to compare them to the amino acids modified in unpolymerized tubulin dimers. Purified bovine tubulin (0.1 mM) was treated with 0.005-0.1 mM chlorpyrifos oxon for 30 min at room temperature and then polymerized by addition of 1 mM GTP to generate microtubules. Microtubules were visualized by atomic force microscopy. Chlorpyrifos oxon-modified residues were identified by tandem ion trap electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry of tryptic peptides. Nanoimaging showed that low concentrations (0.005 and 0.01 mM) of chlorpyrifos oxon yielded short, thin microtubules. A concentration of 0.025 mM stimulated polymerization, while high concentrations (0.05 and 0.1 mM) caused aggregation. Of the 17 tyrosines covalently modified by chlorpyrifos oxon in unpolymerized tubulin dimers, only 2 tyrosines were labeled in polymerized microtubules. The two labeled tyrosines in polymerized tubulin were Tyr 103 in EDAANNYR of alpha tubulin, and Tyr 281 in GSQQYR of beta tubulin. In conclusion, chlorpyrifos oxon binding to tubulin disrupts tubulin polymerization. These results may lead to an understanding of the neurotoxicity of organophosphorus agents.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.