ArticleJournal of molecular biology2009
Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity.
Article in Journal of molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Engineering filamentous myosins for optical control of contractility.bioRxiv : the preprint server for biology · 2025Article
- Power Strokes in Molecular Motors: Predictive, Irrelevant, or Somewhere in Between?Journal of the American Chemical Society · 2025Article
- Motor crosslinking augments elasticity in active nematics.Soft matter · 2024Article
- One must reconstitute the functions of interest from purified proteins.Frontiers in physiology · 2024Review
- Sterically driven current reversal in a molecular motor model.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Antiparallel Coiled-Coil Domain Allows Multiple Forward Step Sizes of Myosin X.The journal of physical chemistry letters · 2023Article
- Optical control of fast and processive engineered myosins in vitro and in living cells.Nature chemical biology · 2021Article
- Controllable molecular motors engineered from myosin and RNA.Nature nanotechnology · 2018Article
- Article
- Vinculin forms a directionally asymmetric catch bond with F-actin.Science (New York, N.Y.) · 2017Article
- Kinematics of the lever arm swing in myosin VI.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- Remote control of myosin and kinesin motors using light-activated gearshifting.Nature nanotechnology · 2014Article
- Engineering myosins for long-range transport on actin filaments.Nature nanotechnology · 2014Article
- Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC).Optics express · 2013Article
- Processive steps in the reverse direction require uncoupling of the lead head lever arm of myosin VI.Molecular cell · 2012Article
- Kinetic properties and small-molecule inhibition of human myosin-6.FEBS letters · 2012Article
- Myosin shifts into reverse gear.Nature nanotechnology · 2012Article
- Engineering controllable bidirectional molecular motors based on myosin.Nature nanotechnology · 2012Article
- Cytoplasmic dynein moves through uncoordinated stepping of the AAA+ ring domains.Science (New York, N.Y.) · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Myosins have diverse mechanical properties reflecting a range of cellular roles. A major challenge is to understand the structural basis for generating novel functions from a common motor core. Myosin VI (M6) is specialized for processive motion toward the (-) end of actin filaments. We have used engineered M6 motors to test and refine the "redirected power stroke" model for (-) end directionality and to explore poorly understood structural requirements for processive stepping. Guided by crystal structures and molecular modeling, we fused artificial lever arms to the catalytic head of M6 at several positions, retaining varying amounts of native structure. We found that an 18-residue alpha-helical insert is sufficient to reverse the directionality of the motor, with no requirement for any calmodulin light chains. Further, we observed robust processive stepping of motors with artificial lever arms, demonstrating that processivity can arise without optimizing lever arm composition or mechanics.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.