ArticleAnalytical biochemistry2009
An adhesion-based method for plasma membrane isolation: evaluating cholesterol extraction from cells and their membranes.
Article in Analytical biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Ascites protects against ferroptosis and enables the peritoneal growth of ovarian cancer.Nature communications · 2026Article
- Harnessing Membrane-Active Peptides for Selective Cancer Targeting: Phosphatidylserine Recognition by Tilapia Piscidin 4.JACS Au · 2026Article
- The Vps13-like protein BLTP2 regulates phosphatidylethanolamine levels to maintain plasma membrane fluidity and breast cancer aggressiveness.Nature cell biology · 2025Article
- Transplants foster B cell alloimmunity by relaying extracellular vesicles to follicular dendritic cells.Cell reports · 2025Article
- Development and Biological Characterization of Cancer Biomimetic Membrane Nanovesicles for Enhancing Therapy Efficacy in Human Glioblastoma Cells.Nanomaterials (Basel, Switzerland) · 2024Article
- Elevated cholesterol in ATAD3 mutants is a compensatory mechanism that leads to membrane cholesterol aggregation.Brain : a journal of neurology · 2024Article
- Microfluidics enabled multi-omics triple-shot mass spectrometry for cell-based therapies.Biomicrofluidics · 2024Article
- Plasma membrane effects of sphingolipid-synthesis inhibition by myriocin in CHO cells: a biophysical and lipidomic study.Scientific reports · 2022Article
- CHO/LY-B cell growth under limiting sphingolipid supply: Correlation between lipid composition and biophysical properties of sphingolipid-restricted cell membranes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Patches and Blebs: A Comparative Study of the Composition and Biophysical Properties of Two Plasma Membrane Preparations from CHO Cells.International journal of molecular sciences · 2020Article
- Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation.Nature communications · 2018Article
- Mutation in S6 domain of HCN4 channel in patient with suspected Brugada syndrome modifies channel function.Pflugers Archiv : European journal of physiology · 2016Article
- Resin embedded multicycle imaging (REMI): a tool to evaluate protein domains.Scientific reports · 2016Article
- Cholesterol segregates into submicrometric domains at the living erythrocyte membrane: evidence and regulation.Cellular and molecular life sciences : CMLS · 2015Article
- Sphingolipid domains in the plasma membranes of fibroblasts are not enriched with cholesterol.The Journal of biological chemistry · 2013Article
- Review on recent advances in the analysis of isolated organelles.Analytica chimica acta · 2012Review
- GREG cells, a dysferlin-deficient myogenic mouse cell line.Experimental cell research · 2012Article
- Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding.Methods in cell biology · 2012Article
- Noninvasive measurements of integrin microclustering under altered membrane cholesterol levels.Biophysical journal · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
A method to isolate large quantities of directly accessible plasma membrane from attached cells is presented. The method is based on the adhesion of cells to an adsorbed layer of polylysine on glass plates, followed by hypotonic lysis with ice-cold distilled water and subsequent washing steps. Optimal conditions for coating glass plates and time for cell attachment were established. No additional chemical or mechanical treatments were used. Contamination of the isolated plasma membrane by cell organelles was less than 5%. The method uses inexpensive, commercially available polylysine and reusable glass plates. Plasma membrane preparations can be made in 15 min. Using this method, we determined that methyl-beta-cyclodextrin differentially extracts cholesterol from fibroblast cells and their plasma membranes and that these differences are temperature dependent. Determination of the cholesterol/phospholipid ratio from intact cells does not reflect methyl-beta-cyclodextrin plasma membrane extraction properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.