ArticlePlanta2009
Dynamics of protein expression during pollen germination in canola (Brassica napus).
Article in Planta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
15 citing papers in PubMed, 43 citations in OpenAlex.
- Heat stress response mechanisms in pollen development.The New phytologist · 2021Review
- Transcriptomic and proteomic analysis reveals mechanisms of low pollen-pistil compatibility during water lily cross breeding.BMC plant biology · 2019Article
- Cytological and Proteomic Analysis of Wheat Pollen Abortion Induced by Chemical Hybridization Agent.International journal of molecular sciences · 2019Article
- Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages.Molecules (Basel, Switzerland) · 2016Article
- Unfolded protein response in pollen development and heat stress tolerance.Plant reproduction · 2016Review
- Bioinformatics resources for pollen.Plant reproduction · 2016Review
- Cytological and Proteomic Analyses of Osmunda cinnamomea Germinating Spores Reveal Characteristics of Fern Spore Germination and Rhizoid Tip Growth.Molecular & cellular proteomics : MCP · 2015Article
- Cytological and proteomic analyses of horsetail (Equisetum arvense L.) spore germination.Frontiers in plant science · 2015Article
- Review
- Evaluation of rice promoters conferring pollen-specific expression in a heterologous system, Arabidopsis.Plant reproduction · 2014Article
- Proteome analysis of the wild and YX-1 male sterile mutant anthers of wolfberry (Lycium barbarum L.).PloS one · 2012Article
- Proteomic analyses of apoplastic proteins from germinating Arabidopsis thaliana pollen.Biochimica et biophysica acta · 2011Article
- Pollen vacuoles and their significance.Planta · 2011Review
- Using proteomics to study sexual reproduction in angiosperms.Sexual plant reproduction · 2011Review
- How to shape a cylinder: pollen tube as a model system for the generation of complex cellular geometry.Sexual plant reproduction · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The proteome of mature (MP) and in vitro germinating pollen (GP) of canola (Brassica napus) were analyzed using the DIGE technology with the objective of identifying proteins and their function in pollen germination. Of the 2,238 protein spots detected in gel images, 344 were differentially expressed in MP and GP samples of which 165 were subjected to MALDI-TOF/TOF and 130 were successfully identified using the NCBInr and Brassica EST databases. The major proteins up-regulated in GP, relative to MP, have roles in carbohydrate metabolism, protein metabolism, and cell wall remodeling. Others with roles in cytoskeleton dynamics, nucleotide and amino acid metabolism, signal transduction, and stress response also showed higher expression in GP. Proteins concerned with transcriptional regulation and ion transport were similar in MP and GP, and some catalases and LEA proteins were down-regulated in GP. A number of proteins including, oleosin, cruciferin, and enolase, were released into the pollen germination medium indicating their potential role in pollen-stigma interaction. Glycosylated proteins were also identified in MP and GP, but their protein profiles were not different. This study has documented the dynamics of protein expression during pollen germination and early tube growth in B. napus and provides insights into the fundamental mechanisms involved in these processes, and in cell growth, cell-cell communication, and cell signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.