ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2009
Longer lasting electroretinographic recordings from the isolated and superfused murine retina.
Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed, 15 citations in OpenAlex.
- Submicromolar copper (II) ions stimulate transretinal signaling in the isolated retina from wild type but not from CaBMC ophthalmology · 2020Article
- Organ Cultures for Retinal Diseases.Frontiers in neuroscience · 2020Review
- Unconjugated bilirubin modulates neuronal signaling only in wild-type mice, but not after ablation of the R-type/CaCNS neuroscience & therapeutics · 2018Article
- Low concentrations of ethanol but not of dimethyl sulfoxide (DMSO) impair reciprocal retinal signal transduction.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2015Article
- Two separate Ni(2+) -sensitive voltage-gated Ca(2+) channels modulate transretinal signalling in the isolated murine retina.Acta ophthalmologica · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAnalysis of retinal signaling in mutant mice has become a powerful tool for studying retinal function and disease. Previous attempts to record from isolated mouse retina have been limited to short time periods (about 90 min). It would be desirable to achieve longer-lasting recordings comparable to those that have been performed in larger vertebrates such as rat, rabbit, cat, and bovine (up to 10 h). We performed a series of recordings from isolated mouse retina under a number of different conditions in order to determine the optimal parameters for this species.
methodsWe used a superfused vertebrate retina assay, for which the murine retina had to be isolated with specific tools. Subsequently, the ERG recordings were optimized for nutrient solution, incubation temperature, and flash light intensity.
resultsTo improve the sensitivity and stability of photoreceptor and retinal network responses from the isolated and superfused murine retina, two different nutrient solutions from rat (physiological Ca(2+)) and bovine (reduced Ca(2+) but increased phosphate buffering capacity) were used. Further, a temperature reduced to 27.5 degrees C, a light intensity ten-fold increased (63 mlux), and an increased flow rate (2 ml/min) provided conditions under which the b-wave response was stable for more than 3 hours. Well-known Ca(2+) channel antagonists (isradipine and NiCl(2)) were tested for their potency to antagonize transretinal signalling.
conclusionIn conclusion, the isolated murine retina can be used as a pharmacological testing system, which provides the additional advantage of selective gene inactivation for better understanding of retinal signalling.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.