ArticleCurrent genetics2009
Development of transformation system for Trichophyton rubrum by electroporation of germinated conidia.
Article in Current genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- A PEG-mediated protoplast transformation system for EGFP labeling of the shot-hole pathogen Wilsonomyces carpophilus.Frontiers in microbiology · 2026Article
- Establishment of a genetic transformation system for cordycipitoid fungusFrontiers in microbiology · 2024Article
- Article
- Metabolomic analysis of Trichophyton rubrum and Microsporum canis during keratin degradation.Scientific reports · 2021Article
- Reference genes for accurate evaluation of expression levels in Trichophyton interdigitale grown under different carbon sources, pH levels and phosphate levels.Scientific reports · 2019Article
- Selection and validation of reference genes for qRT-PCR analysis of gene expression in Microsporum canis growing under different adhesion-inducing conditions.Scientific reports · 2018Article
- Genetic Manipulations in Dermatophytes.Mycopathologia · 2017Review
- Protoplast transformation as a potential platform for exploring gene function in Verticillium dahliae.BMC biotechnology · 2016Article
- Transient transformation of Podosphaera xanthii by electroporation of conidia.BMC microbiology · 2015Article
- Transformation of Epichloë typhina by electroporation of conidia.BMC research notes · 2011Article
- Sequenced dermatophyte strains: growth rate, conidiation, drug susceptibilities, and virulence in an invertebrate model.Fungal genetics and biology : FG & B · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dermatophytes are the fungi that can cause infections of skin, hair, and nails due to their ability to utilize keratin. The genetic transformation systems of dermatophytes were successfully applied to Trichophyton mentagrophytes and Microsporum canis. Here we describe the procedure for genetic transformation of Trichophyton rubrum by electroporation of their germinated conidia. A linearized transformation vector (pCHSH75-Pch/GFP/TtrpC) containing bacterial hygromycin B phosphotransferase gene (hph) and green fluorescent protein gene (egfp) was introduced into the germinated conidia of T. rubrum by electroporation. PCR reaction analysis showed that egfp gene was integrated randomly and Southern blotting analysis demonstrated a single integration of hph gene into the chromosomal DNA of randomly selected transformant. In this work we report the efficient transformation and selection of the stable T. rubrum transformants.
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