ArticleInvestigative ophthalmology & visual science2009
Delivery of antioxidant enzyme genes to protect against ischemia/reperfusion-induced injury to retinal microvasculature.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- A dual-responsive CO-releasing nanogel ameliorates retinal ischemia-reperfusion injury by restoring mitochondrial homeostasis and attenuating cGAS-STING pathway activation.Materials today. Bio · 2026Article
- Catalytic neural stem cell exosomes for multi-stage targeting and synergistical therapy of retinal ischemia-reperfusion injury.Cell reports. Medicine · 2025Article
- Redefining cell death: ferroptosis as a game-changer in ophthalmology.Frontiers in immunology · 2025Review
- Role of Flavonoids in the Treatment of Iron Overload.Frontiers in cell and developmental biology · 2021Review
- Retinal ganglion cell complex and peripapillary retinal nerve fiber layer thicknesses following carotid endarterectomy.International ophthalmology · 2019Article
- Echinacoside protects retinal ganglion cells from ischemia/reperfusion-induced injury in the rat retina.Molecular vision · 2018Article
- Article
- Timing of Antioxidant Gene Therapy: Implications for Treating Dry AMD.Investigative ophthalmology & visual science · 2017Article
- The Involvement ofJournal of ophthalmology · 2017Article
- Arginase 2 promotes neurovascular degeneration during ischemia/reperfusion injury.Cell death & disease · 2016Article
- Adeno-associated virus mediated SOD gene therapy protects the retinal ganglion cells from chronic intraocular pressure elevation induced injury via attenuating oxidative stress and improving mitochondrial dysfunction in a rat model.American journal of translational research · 2016Article
- Effects of antioxidant gene therapy on the development of diabetic retinopathy and the metabolic memory phenomenon.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2015Article
- Coenzyme Q10 ameliorates oxidative stress and prevents mitochondrial alteration in ischemic retinal injury.Apoptosis : an international journal on programmed cell death · 2014Article
- Minocycline prevents retinal inflammation and vascular permeability following ischemia-reperfusion injury.Journal of neuroinflammation · 2013Article
- Vitreous mediators in retinal hypoxic diseases.Mediators of inflammation · 2013Review
- Hypoxia-induced oxidative stress in ischemic retinopathy.Oxidative medicine and cellular longevity · 2012Review
- Nrf2 has a protective role against neuronal and capillary degeneration in retinal ischemia-reperfusion injury.Free radical biology & medicine · 2011Article
- Targets of Neuroprotection in Glaucoma.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and TherapeuticsReview
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
purposeRetinal ischemia/reperfusion (I/R) injury results in the generation of reactive oxygen species (ROS). The aim of this study was to investigate whether delivery of the manganese superoxide dismutase gene (SOD2) or the catalase gene (CAT) could rescue the retinal vascular damage induced by I/R in mice.
methodsI/R injury to the retina was induced in mice by elevating intraocular pressure for 2 hours, and reperfusion was established immediately afterward. One eye of each mouse was pretreated with plasmids encoding manganese superoxide dismutase or catalase complexed with cationic liposomes and delivered by intravitreous injection 48 hours before initiation of the procedure. Superoxide ion, hydrogen peroxide, and 4-hydroxynonenal (4-HNE) protein modifications were measured by fluorescence staining, immunohistochemistry, and Western blot analysis 1 day after the I/R injury. At 7 days after injury, retinal vascular cell apoptosis and acellular capillaries were quantitated.
resultsSuperoxide ion, hydrogen peroxide, and 4-HNE protein modifications increased at 24 hours after I/R injury. Administration of plasmids encoding SOD2 or CAT significantly reduced levels of superoxide ion, hydrogen peroxide, and 4-HNE. Retinal vascular cell apoptosis and acellular capillary numbers increased greatly by 7 days after the injury. Delivery of SOD2 or CAT inhibited the I/R-induced apoptosis of retinal vascular cell and retinal capillary degeneration.
conclusionsDelivery of antioxidant genes inhibited I/R-induced retinal capillary degeneration, apoptosis of vascular cells, and ROS production, suggesting that antioxidant gene therapy might be a treatment for I/R-related disease.
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