ArticleJournal of experimental botany2009
Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response.
Article in Journal of experimental botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 143 papers.
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Who cites it
143 citing papers in PubMed, 317 citations in OpenAlex.
- Anther and pollen thermotolerance: molecular mechanisms and implications for plant reproduction under heat stress.Planta · 2026Review
- A Review of Differential Plant Responses to Drought, Heat, and Combined Drought + Heat Stress.Current issues in molecular biology · 2025Review
- Thermotolerance and post-fire growth in Rhizina undulata is associated with the expansion of heat stress-related protein families.BMC genomics · 2025Article
- Comparative transcriptome analysis reveals the role of sugar signaling in response to high temperature stress in Armillaria gallica.BMC microbiology · 2025Article
- Heat-stable protein PGSL1 enhances pollen germination and tube growth at high temperature.Nature communications · 2025Article
- Article
- Screening methods for thermotolerance in pollen.Annals of botany · 2025Review
- Unraveling the role of autophagy and antioxidants in anther and pistil responses to heat stress in rapeseed (Brassica napus L.).Plant cell reports · 2025Article
- Enhanced pollen tube performance at high temperature contributes to thermotolerant fruit and seed production in tomato.Current biology : CB · 2024Article
- Flavonols improve tomato pollen thermotolerance during germination and tube elongation by maintaining reactive oxygen species homeostasis.The Plant cell · 2024Article
- Pollen viability, longevity, and function in angiosperms: key drivers and prospects for improvement.Plant reproduction · 2024Review
- First Peek into the Transcriptomic Response in Heat-Stressed Tomato Inoculated withPlants (Basel, Switzerland) · 2024Article
- Enhanced pollen tube performance at high temperature contributes to thermotolerant fruit production in tomato.bioRxiv : the preprint server for biology · 2024Article
- Intraspecific variation in responses to extreme and moderate temperature stress in the wild species,AoB PLANTS · 2024Article
- Elevated Temperature Effects on Protein Turnover Dynamics inInternational journal of molecular sciences · 2024Article
- Deleterious Effects of Heat Stress on the Tomato, Its Innate Responses, and Potential Preventive Strategies in the Realm of Emerging Technologies.Metabolites · 2024Review
- Appraisal of the Role of Gaseous Signaling Molecules in Thermo-Tolerance Mechanisms in Plants.Plants (Basel, Switzerland) · 2024Review
- Discovering the regulators of heat stress tolerance inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Comparative transcriptome profiling of contrasting finger millet (Eleusine coracana (L.) Gaertn) genotypes under heat stress.Molecular biology reports · 2024Article
- Variability of plant transcriptomic responses under stress acclimation: a review from high throughput studies.Acta biochimica Polonica · 2024Review
83 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Above-optimal temperatures reduce yield in tomato largely because of the high heat stress (HS) sensitivity of the developing pollen grains. The high temperature response, especially at this most HS-sensitive stage of the plant, is poorly understood. To obtain an overview of molecular mechanisms underlying the HS response (HSR) of microspores, a detailed transcriptomic analysis of heat-stressed maturing tomato microspores was carried out using a combination of Affymetrix Tomato Genome Array and cDNA-amplified fragment length polymorphism (AFLP) techniques. The results were corroborated by reverse transcription-PCR (RT-PCR) and immunoblot analyses. The data obtained reveal the involvement of specific members of the small heat shock protein (HSP) gene family, HSP70 and HSP90, in addition to the HS transcription factors A2 (HSFA2) and HSFA3, as well as factors other than the classical HS-responsive genes. The results also indicate HS regulation of reactive oxygen species (ROS) scavengers, sugars, plant hormones, and regulatory genes that were previously implicated in other types of stress. The use of cDNA-AFLP enabled the detection of genes representing pollen-specific functions that are missing from the tomato Affymetrix chip, such as those involved in vesicle-mediated transport and a pollen-specific, calcium-dependent protein kinase (CDPK2). For several genes, including LeHSFA2, LeHSP17.4-CII, as well as homologues of LeHSP90 and AtVAMP725, higher basal expression levels were detected in microspores of cv. Hazera 3042 (a heat-tolerant cultivar) compared with microspores of cv. Hazera 3017 (a heat-sensitive cultivar), marking these genes as candidates for taking part in microspore thermotolerance. This work provides a comprehensive analysis of the molecular events underlying the HSR of maturing microspores of a crop plant, tomato.
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