ArticleBiosensors & bioelectronics2009
Quantification of DNA and protein adsorption by optical phase shift.
Article in Biosensors & bioelectronics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Multi-parametric interferometric reflectance imaging sensor.Scientific reports · 2026Article
- Affinity-Based Copolymer Coating for Oriented Protein Immobilization in Biosensor Development.Biosensors · 2025Article
- Solid-Phase Optical Sensing Techniques for Sensitive Virus Detection.Sensors (Basel, Switzerland) · 2023Review
- Highly-Sensitive, Label-Free Detection of Microorganisms and Viruses via Interferometric Reflectance Imaging Sensor.Micromachines · 2023Review
- Attomolar sensitivity microRNA detection using real-time digital microarrays.Scientific reports · 2022Article
- Active Opto-Magnetic Biosensing with Silicon Microring Resonators.Sensors (Basel, Switzerland) · 2022Article
- CovidArray: A Microarray-Based Assay with High Sensitivity for the Detection of Sars-Cov-2 in Nasopharyngeal Swabs.Sensors (Basel, Switzerland) · 2021Article
- Bulk-Effect-Free Method for Binding Kinetic Measurements Enabling Small-Molecule Affinity Characterization.ACS omega · 2021Article
- Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein.Biosensors · 2020Article
- Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects.Analytical and bioanalytical chemistry · 2020Review
- Membrane-binding peptides for extracellular vesicles on-chip analysis.Journal of extracellular vesicles · 2020Article
- Interferometric Reflectance Imaging Sensor (IRIS)--A Platform Technology for Multiplexed Diagnostics and Digital Detection.Sensors (Basel, Switzerland) · 2015Review
- Article
- Integrated imaging instrument for self-calibrated fluorescence protein microarrays.The Review of scientific instruments · 2013Article
- Single nanoparticle detection for multiplexed protein diagnostics with attomolar sensitivity in serum and unprocessed whole blood.Analytical chemistry · 2013Article
- Label-free microcavity biosensors: steps towards personalized medicine.Sensors (Basel, Switzerland) · 2012Article
- TATA binding proteins can recognize nontraditional DNA sequences.Biophysical journal · 2012Article
- Interferometric methods for label-free molecular interaction studies.Analytical chemistry · 2012Review
- Multiplexed method to calibrate and quantitate fluorescence signal for allergen-specific IgE.Analytical chemistry · 2011Article
- Synergetic chemiluminescence and label-free dual detection for developing a hepatitis protein array.Journal of immunological methods · 2011Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
A primary advantage of label-free detection methods over fluorescent measurements is its quantitative detection capability, since an absolute measure of adsorbed material facilitates kinetic characterization of biomolecular interactions. Interferometric techniques relate the optical phase to biomolecular layer density on the surface, but the conversion factor has not previously been accurately determined. We present a calibration method for phase shift measurements and apply it to surface-bound bovine serum albumin, immunoglobulin G, and single-stranded DNA. Biomolecules with known concentrations dissolved in salt-free water were spotted with precise volumes on the array surface and upon evaporation of the water, left a readily calculated mass. Using our label-free technique, the calculated mass of the biolayer was compared with the measured thickness, and we observed a linear dependence over 4 orders of magnitude. We determined that the widely accepted conversion of 1 nm of thickness corresponds to approximately 1 ng/mm(2) surface density held reasonably well for these substances and through our experiments can now be further specified for different types of biomolecules. Through accurate calibration of the dependence of thickness on surface density, we have established a relation allowing precise determination of the absolute number of molecules for single-stranded DNA and two different proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.