ArticleSurgery2009
Proteome and system ontology of hemorrhagic shock: exploring early constitutive changes in postshock mesenteric lymph.
Article in Surgery, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 44 citations in OpenAlex.
- Plasma first in the field for postinjury hemorrhagic shock.Shock (Augusta, Ga.) · 2014Trial
- Protein expression profiles of human lymph and plasma mapped by 2D-DIGE and 1D SDS-PAGE coupled with nanoLC-ESI-MS/MS bottom-up proteomics.Journal of proteomics · 2013Trial
- Article
- The Gut-Lung Axis in Systemic Inflammation. Role of Mesenteric Lymph as a Conduit.American journal of respiratory cell and molecular biology · 2021Review
- Contribution of the plasma and lymph Degradome and Peptidome to the MHC Ligandome.Immunogenetics · 2019Review
- Precious cargo: Modulation of the mesenteric lymph exosome payload after hemorrhagic shock.The journal of trauma and acute care surgery · 2019Article
- Lymphatic Vessel Network Structure and Physiology.Comprehensive Physiology · 2018Review
- The Metabolopathy of Tissue Injury, Hemorrhagic Shock, and Resuscitation in a Rat Model.Shock (Augusta, Ga.) · 2018Article
- Peptidomic Analysis of Rat Plasma: Proteolysis in Hemorrhagic Shock.Shock (Augusta, Ga.) · 2016Article
- The Dendritic Cell Major Histocompatibility Complex II (MHC II) Peptidome Derives from a Variety of Processing Pathways and Includes Peptides with a Broad Spectrum of HLA-DM Sensitivity.The Journal of biological chemistry · 2016Article
- A "CLEAN CASE" OF SYSTEMIC INJURY: MESENTERIC LYMPH AFTER HEMORRHAGIC SHOCK ELICITS A STERILE INFLAMMATORY RESPONSE.Shock (Augusta, Ga.) · 2015Article
- TACTIC: Trans-Agency Consortium for Trauma-Induced Coagulopathy.Journal of thrombosis and haemostasis : JTH · 2015Review
- Pathologic metabolism: an exploratory study of the plasma metabolome of critical injury.The journal of trauma and acute care surgery · 2015Article
- Lymph is not a plasma ultrafiltrate: a proteomic analysis of injured patients.Shock (Augusta, Ga.) · 2014Article
- Dynamic changes in rat mesenteric lymph proteins following trauma using label-free mass spectrometry.Shock (Augusta, Ga.) · 2014Article
- Redefining the gut as the motor of critical illness.Trends in molecular medicine · 2014Review
- Intestine-specific deletion of microsomal triglyceride transfer protein increases mortality in aged mice.PloS one · 2014Article
- Article
- The lymph self-antigen repertoire.Frontiers in immunology · 2013Review
- Carrying yourself: self antigen composition of the lymphatic fluid.Lymphatic research and biology · 2013Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundPostshock mesenteric lymph (PSML) is the mechanistic link between splanchnic ischemia reperfusion (IR) and remote organ injury. We hypothesize that an unbiased inspection of the proteome of PSML will reveal previously unrecognized aberrations in systems biology provoked by hemorrhage-induced mesenteric IR injury in vivo.
methodsShock was induced in male Sprague-Dawley rats by controlled hemorrhage, and the mesenteric duct was cannulated for lymph collection. Preshock and postshock lymph were collected for differential in-gel electrophoresis (DIGE)-based proteomics. Proteins that increased or decreased in relative concentration > or =1.5-fold were selected for trypsin digestion and analysis by mass spectrometry (MS).
resultsEvidence of tissue injury was detected by an increase in cell/tissue proteins in PSML. Components of coagulation were depleted, whereas products of hemolysis were increased. Haptoglobin was decreased, which supports an early postshock hemolytic process. Interestingly, several protective protease inhibitors were decreased in PSML. The unexpected findings were an increase in alpha-enolase (a key glycolitic enzyme and cell-surface plasminogen binding receptor, +2.4-fold change) and increased major urinary protein (MUP, a sex-specific lipid-binding protein, +17.1-fold change) in PSML.
conclusionA proteomic evaluation of PSML revealed evidence of several shock-associated processes: protein release from tissue injury, depletion of coagulation factors and evidence of hemolysis, depletion of protective protease inhibitors, and an increase in abundance of lipid carriers. These results suggest that constitutive changes in the proteome of PSML may provide novel insights into the complex pathophysiology of postshock systems biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.