ArticleMolecular microbiology2009
Cytoplasmic membrane protonmotive force energizes periplasmic interactions between ExbD and TonB.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Primary role of the Tol-Pal complex in bacterial outer membrane lipid homeostasis.Nature communications · 2025Article
- Substrate Uptake by TonB-Dependent Outer Membrane Transporters.Molecular microbiology · 2024Review
- Energization of Outer Membrane Transport by the ExbB ExbD Molecular Motor.Journal of bacteriology · 2023Review
- Genome-Wide Screening of Oxidizing Agent Resistance Genes inAntioxidants (Basel, Switzerland) · 2021Article
- Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.Chemical reviews · 2021Review
- Vitamin BBiophysical journal · 2021Article
- Extracellular loops of BtuB facilitate transport of vitamin B12 through the outer membrane of E. coli.PLoS computational biology · 2020Article
- The Intrinsically Disordered Region of ExbD Is Required for Signal Transduction.Journal of bacteriology · 2020Article
- Evolution of the Stator Elements of Rotary Prokaryote Motors.Journal of bacteriology · 2020Review
- Two novel proteins, TtpB2 and TtpD2, are essential for iron transport in the TonB2 system of Vibrio vulnificus.MicrobiologyOpen · 2020Article
- Article
- Stenotrophomonas maltophilia produces an EntC-dependent catecholate siderophore that is distinct from enterobactin.Microbiology (Reading, England) · 2017Article
- Survival of Helicobacter pylori in gastric acidic territory.Helicobacter · 2017Review
- Going Outside the TonB Box: Identification of Novel FepA-TonB InteractionsJournal of bacteriology · 2017Article
- Desert Dust as a Source of Iron to the Globally Important DiazotrophFrontiers in microbiology · 2017Article
- Article
- Article
- From Homodimer to Heterodimer and Back: Elucidating the TonB Energy Transduction Cycle.Journal of bacteriology · 2015Article
- Membrane Protein Complex ExbB4-ExbD1-TonB1 from Escherichia coli Demonstrates Conformational Plasticity.Journal of bacteriology · 2015Article
- The putative Poc complex controls two distinct Pseudomonas aeruginosa polar motility mechanisms.Molecular microbiology · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The TonB system of Escherichia coli (TonB/ExbB/ExbD) transduces the protonmotive force (pmf) of the cytoplasmic membrane to drive active transport by high-affinity outer membrane transporters. In this study, chromosomally encoded ExbD formed formaldehyde-linked complexes with TonB, ExbB and itself (homodimers) in vivo. Pmf was required for detectable cross-linking between TonB-ExbD periplasmic domains. Consistent with that observation, the presence of inactivating transmembrane domain mutations ExbD(D25N) or TonB(H20A) also prevented efficient formaldehyde cross-linking between ExbD and TonB. A specific site of periplasmic interaction occurred between ExbD(A92C) and TonB(A150C) and required functional transmembrane domains in both proteins. Conversely, neither TonB, ExbB nor pmf were required for ExbD dimer formation. These data suggest two possible models where either dynamic complex formation occurred through transmembrane domains or the transmembrane domains of ExbD and TonB configure their respective periplasmic domains. Analysis of T7-tagged ExbD with anti-ExbD antibodies revealed that a T7 tag was responsible both for our previous failure to detect T7-ExbD-ExbB and T7-ExbD-TonB formaldehyde-linked complexes and for the concomitant artefactual appearance of T7-ExbD trimers.
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Registered trials
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