ArticleAnalytical chemistry2009
Capturing single molecules of immunoglobulin and ricin with an aptamer-encoded glass nanopore.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed.
- Label-Free Profiling of Immunoglobulin Isotypes Using Solid-State Nanopores.Small methods · 2026Article
- Noninvasive methods to monitor dynamic single-cell events.Trends in cell biology · 2026Review
- Nanopore Environmental Analysis.JACS Au · 2025Review
- Solid-State Nanopores for Biomolecular Analysis and Detection.Advances in biochemical engineering/biotechnology · 2024Review
- Article
- An ultrasensitive aptasensor of SARS-CoV-2 N protein based on ion current rectification with nanopipettes.Sensors and actuators. B, Chemical · 2023Article
- High throughput isolation of RNA from single-cells within an intact tissue for spatial and temporal sequencing a reality.PloS one · 2023Article
- Nanopore sensors for viral particle quantification: current progress and future prospects.Bioengineered · 2021Review
- [Highly toxic type Ⅱ ribosome-inactivating proteins ricin and abrin and their detection methods: a review].Se pu = Chinese journal of chromatography · 2021Review
- Sensing with Nanopores and Aptamers: A Way Forward.Sensors (Basel, Switzerland) · 2020Review
- Aptamers against Immunoglobulins: Design, Selection and Bioanalytical Applications.International journal of molecular sciences · 2020Review
- Wireless nanopore electrodes for analysis of single entities.Nature protocols · 2019Article
- Review
- Hydrogen Peroxide Sensing Based on Inner Surfaces Modification of Solid-State Nanopore.Nanoscale research letters · 2017Article
- Selective single molecule nanopore sensing of proteins using DNA aptamer-functionalised gold nanoparticles.Chemical science · 2017Article
- Resistive-pulse and rectification sensing with glass and carbon nanopipettes.Proceedings. Mathematical, physical, and engineering sciences · 2017Review
- Enhanced Temporal Resolution with Ion Channel-Functionalized Sensors Using a Conductance-Based Measurement Protocol.Analytical chemistry · 2017Article
- Tuning the selectivity and sensitivity of an OmpG nanopore sensor by adjusting ligand tether length.ACS sensors · 2016Article
- Selective Detection of Protein Homologues in Serum Using an OmpG Nanopore.Analytical chemistry · 2015Article
- Electrostatic Interactions between OmpG Nanopore and Analyte Protein Surface Can Distinguish between Glycosylated Isoforms.The journal of physical chemistry. B · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Nanopore-based single-molecule biosensors have been extensively studied. Protein pores that have receptors attached to them are target-selective, but their real-time applications are limited by the fragility of the lipid membrane into which the protein pores are embedded. Synthetic nanopores are more stable and provide flexible pore sizes, but the selectivity is low when detecting in the translocation mode. In spite of modifications with probing molecules, such as antibodies, to potentiate specific targeting, these nanopores fail to bind individual target molecules. Distinguishing between binding and translocation blocks remains unsolved. Here, we propose an aptamer-encoded nanopore that overcomes these challenges. Aptamers are well-known probing oligonucleotides that have high sensitivity and selectivity. In contrast to antibodies, aptamers are much smaller than their targets, rendering target blockades in the nanopore much more distinguishable. We used aptamer-encoded nanopores to detect single molecules of immunoglobulin E and the bioterrorist agent ricin, sequentially captured by the immobilized aptamer in the sensing zone of the pore. The functional nanopore also probed sequence-dependent aptamer-protein interactions. These findings will facilitate the development of a universal nanopore for multitarget detection.
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Registered trials
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