ArticleMicrocirculation (New York, N.Y. : 1994)2009
Methods for lymphatic vessel culture and gene transfection.
Article in Microcirculation (New York, N.Y. : 1994), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 34 citations in OpenAlex.
- Lymphangion-chip: a microphysiological system which supports co-culture and bidirectional signaling of lymphatic endothelial and muscle cells.Lab on a chip · 2021Article
- Lymphatic Cannulation for Lymph Sampling and Molecular Delivery.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Prolonged intake of desloratadine: mesenteric lymphatic vessel dysfunction and development of obesity/metabolic syndrome.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- Differences in L-type CaAmerican journal of physiology. Heart and circulatory physiology · 2018Article
- Temporal Dynamics of the Rat Thoracic Duct Contractility in the Presence of Imposed Flow.Lymphatic research and biology · 2017Article
- Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm's Canal Reporter Rat.Scientific reports · 2017Article
- Histamine as an Endothelium-Derived Relaxing Factor in Aged Mesenteric Lymphatic Vessels.Lymphatic research and biology · 2017Article
- The pro-inflammatory cytokine TNF-α inhibits lymphatic pumping via activation of the NF-κB-iNOS signaling pathway.Microcirculation (New York, N.Y. : 1994) · 2017Article
- A lumped parameter model of mechanically mediated acute and long-term adaptations of contractility and geometry in lymphatics for characterization of lymphedema.Biomechanics and modeling in mechanobiology · 2016Article
- Article
- In vivo visualization and quantification of collecting lymphatic vessel contractility using near-infrared imaging.Scientific reports · 2016Article
- Effects of dynamic shear and transmural pressure on wall shear stress sensitivity in collecting lymphatic vessels.American journal of physiology. Regulatory, integrative and comparative physiology · 2015Article
- Mechanobiological oscillators control lymph flow.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- [Influence of 7-dehydrocholesterol reductase gene silencing on the fusion of mouse palatal shelves].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2015Article
- Imaging the lymphatic system.Microvascular research · 2014Review
- Involvement of histamine in endothelium-dependent relaxation of mesenteric lymphatic vessels.Microcirculation (New York, N.Y. : 1994) · 2014Article
- Maximum shortening velocity of lymphatic muscle approaches that of striated muscle.American journal of physiology. Heart and circulatory physiology · 2013Article
- Use of a PEG-conjugated bright near-infrared dye for functional imaging of rerouting of tumor lymphatic drainage after sentinel lymph node metastasis.Biomaterials · 2013Article
- Genetic removal of basal nitric oxide enhances contractile activity in isolated murine collecting lymphatic vessels.The Journal of physiology · 2013Article
- Intrinsic increase in lymphangion muscle contractility in response to elevated afterload.American journal of physiology. Heart and circulatory physiology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
Abstract
objectiveTo develop the techniques needed for the specific gene/protein targeting transfection experiments in isolated lymphatic vessels, we completed two major tasks: 1) optimize the experimental conditions to maintain the viability of isolated rat lymphatic vessels in culture for sufficiently long periods of time to permit knockdown or overexpression of selected proteins/genes and 2) develop effective transfection protocols for lymphatic muscle and endothelial cells in intact lymphatic vessels without nonspecific impairment of lymphatic contractile function due to the transfection protocol itself.
methodsExperimental protocols were developed for the maintenance of isolated lymphatic vessels under nonpressurized and pressurized conditions for 3-12 days in culture and for adenoviral gene transfection of the lymphatic muscle and endothelial cells.
resultsThe data demonstrate the effectiveness of the newly developed experimental protocols for the maintenance of isolated rat mesenteric lymphatic vessels and thoracic duct in culture up to 3-12 days without significant impairment of the parameters of their pumping and effective adenoviral/GFP transfection of lymphatic endothelial and muscle cells in isolated rat mesenteric lymphatic vessels.
conclusionsThese experimental techniques will extend the set of the modern experimental tools available to researchers investigating the physiology of lymphatic function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.