ArticleEuropean biophysics journal : EBJ2009
Using a five-state model for fitting amplitude histograms from MaxiK channels: beta-distributions reveal more than expected.
Article in European biophysics journal : EBJ, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed.
- Similarity of the ion occupations of KChannels (Austin, Tex.) · 2026Article
- Binding kinetics of quaternary ammonium ions in Kcv potassium channels.Channels (Austin, Tex.) · 2024Article
- Asymmetric Interplay Between KFrontiers in physiology · 2021Article
- Extended beta distributions open the access to fast gating in bilayer experiments-assigning the voltage-dependent gating to the selectivity filter.FEBS letters · 2017Article
- How to resolve microsecond current fluctuations in single ion channels: the power of beta distributions.Channels (Austin, Tex.) · 2015Review
- Lack of negative slope in I-V plots for BK channels at positive potentials in the absence of intracellular blockers.The Journal of general physiology · 2013Article
- Ca2+ block and flickering both contribute to the negative slope of the IV curve in BK channels.The Journal of general physiology · 2013Article
- Modulation of enrofloxacin binding in OmpF by Mg2+ as revealed by the analysis of fast flickering single-porin current.The Journal of general physiology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fast gating of ion channels with rate constants higher than the corner frequency of the recording set-up can be evaluated by fitting so-called beta distributions to measured amplitude histograms. Up to now, this was preferentially done for O-C Markov sub-models with one open and one closed state. Here, a fit of the amplitude histograms from MaxiK (BK) single-channel records was achieved with a five-state model with two open and three closed states including three open-close transitions with rate constants higher than the corner frequency (20 kHz) of the inevitable low-pass filter of the recording system. The numerical values of the rate constants of these transitions enabled a nearly one-to-one relationship between typical regions of the histograms and the reactions in the Markov model. These characteristic features are the width of the peak at the apparent single-channel current, the side slopes at the open and at the closed peak, and the depth of the valley between the two peaks. However, the simplex routine alone was incapable of finding the solution but could do so if guided by hand along a suggested strategy.
Indexed as
Identifiers
19626320What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.