ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2009
Submillisecond optical reporting of membrane potential in situ using a neuronal tracer dye.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 99 citations in OpenAlex.
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- High-speed voltage imaging of action potentials in molecular layer interneurons reveals sensory-driven synchrony that augments movement.Cell reports · 2025Article
- Molecular determinants of [Formula: see text] and cation transport in the human cation-dependent ClAmerican journal of physiology. Cell physiology · 2025Article
- Advances in two-photon imaging for monitoring neural activity in behaving mice.Frontiers in neuroscience · 2025Review
- Fxr1 Deletion from Cortical Parvalbumin Interneurons Modifies Their Excitatory Synaptic Responses.eNeuro · 2025Article
- Inter and intralaminar excitation of parvalbumin interneurons in mouse barrel cortex.PloS one · 2024Article
- Velocity of conduction between columns and layers in barrel cortex reported by parvalbumin interneurons.Cerebral cortex (New York, N.Y. : 1991) · 2023Article
- Inter and Intralaminar Excitation of Parvalbumin Interneurons in Mouse Barrel Cortex.bioRxiv : the preprint server for biology · 2023Article
- Imaging Voltage Globally and in Isofrequency Lamina in Slices of Mouse Ventral Cochlear Nucleus.eNeuro · 2023Article
- Fluorescence Imaging of Cell Membrane Potential: From Relative Changes to Absolute Values.International journal of molecular sciences · 2023Review
- Morphology and Dendrite-Specific Synaptic Properties of Midbrain Neurons Shape Multimodal Integration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Optical probing of local membrane potential with fluorescent polystyrene beads.Biophysical reports · 2021Article
- Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs.Biophysical reports · 2021Article
- A dark quencher genetically encodable voltage indicator (dqGEVI) exhibits high fidelity and speed.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Insights Into Spinal Dorsal Horn Circuit Function and Dysfunction Using Optical Approaches.Frontiers in neural circuits · 2020Review
- Optical Studies of Action Potential Dynamics with hVOS probes.Current opinion in biomedical engineering · 2019Article
- Spying on Neuronal Membrane Potential with Genetically Targetable Voltage Indicators.Journal of the American Chemical Society · 2019Article
- Visualization of Dynamic Sub-microsecond Changes in Membrane Potential.Biophysical journal · 2019Article
- Tethered Bichromophoric Fluorophore Quencher Voltage Sensitive Dyes.ACS sensors · 2018Article
- Relative positioning of Kv11.1 (hERG) KScientific reports · 2018Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
A major goal in neuroscience is the development of optical reporters of membrane potential that are easy to use, have limited phototoxicity, and achieve the speed and sensitivity necessary for detection of individual action potentials in single neurons. Here we present a novel, two-component optical approach that attains these goals. By combining DiO, a fluorescent neuronal tracer dye, with dipicrylamine (DPA), a molecule whose membrane partitioning is voltage-sensitive, optical signals related to changes in membrane potential based on FRET (Förster resonance energy transfer) are reported. Using DiO/DPA in HEK-293 cells with diffraction-limited laser spot illumination, depolarization-induced fluorescence changes of 56% per 100 mV (tau approximately 0.1 ms) were obtained, while in neuronal cultures and brain slices, action potentials (APs) generated a Delta F/F per 100 mV of >25%. The high sensitivity provided by DiO/DPA enabled the detection of subthreshold activity and high-frequency APs in single trials from somatic, axonal, or dendritic membrane compartments. Recognizing that DPA can depress excitability, we assayed the amplitude and duration of single APs, burst properties, and spontaneous firing in neurons of primary cultures and brain slices and found that they are undetectably altered by up to 2 microm DPA and only slightly perturbed by 5 microm DPA. These findings substantiate a simple, noninvasive method that relies on a neuronal tracer dye for monitoring electrical signal flow, and offers unique flexibility for the study of signaling within intact neuronal circuits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.