ArticleBiomaterials2009
Skeletal muscle tissue engineering: a maturation model promoting long-term survival of myotubes, structural development of the excitation-contraction coupling apparatus and neonatal myosin heavy chain expression.
Article in Biomaterials, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Development of a functional sarcopenia model utilizing a microcantilever microphysiological system as a phenotypic disease model.In vitro models · 2026Article
- Short-Stranded Zein Fibers for Muscle Tissue Engineering in Alginate-Based Composite Hydrogels.Gels (Basel, Switzerland) · 2023Article
- Mimicking the Tendon Microenvironment to Enhance Skeletal Muscle Adhesion and Longevity in a Functional Microcantilever Platform.ACS biomaterials science & engineering · 2023Article
- Design of Recombinant Spider Silk Proteins for Cell Type Specific Binding.Advanced healthcare materials · 2023Article
- Long-Term High-Density Extracellular Recordings Enable Studies of Muscle Cell Physiology.Frontiers in physiology · 2018Article
- Functional analysis of human intrafusal fiber innervation by human γ-motoneurons.Scientific reports · 2017Article
- Self-contained, low-cost Body-on-a-Chip systems for drug development.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- Tissue-Engineered Larynx: Future Applications in Laryngeal Cancer.Current otorhinolaryngology reports · 2017Review
- On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes.Scientific reports · 2016Article
- Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.Nature protocols · 2016Article
- A system for studying mechanisms of neuromuscular junction development and maintenance.Development (Cambridge, England) · 2016Article
- Spatial Geometries of Self-Assembled Chitohexaose Monolayers Regulate Myoblast Fusion.International journal of molecular sciences · 2016Article
- Myoprotective effects of bFGF on skeletal muscle injury in pressure-related deep tissue injury in rats.Burns & trauma · 2016Article
- Interactions between Skeletal Muscle Myoblasts and their Extracellular Matrix Revealed by a Serum Free Culture System.PloS one · 2015Article
- Myogenic progenitors and imaging single-cell flow analysis: a model to study commitment of adult muscle stem cells.Journal of muscle research and cell motility · 2014Article
- Utilization of microscale silicon cantilevers to assess cellular contractile function in vitro.Journal of visualized experiments : JoVE · 2014Article
- How multi-organ microdevices can help foster drug development.Advanced drug delivery reviews · 2014Review
- In vitro Differentiation of Functional Human Skeletal Myotubes in a Defined System.Biomaterials science · 2014Article
- Myelination and node of Ranvier formation on sensory neurons in a defined in vitro system.In vitro cellular & developmental biology. Animal · 2013Article
- Correlation of embryonic skeletal muscle myotube physical characteristics with contractile force generation on an atomic force microscope-based bio-microelectromechanical systems device.Applied physics letters · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The use of defined in vitro systems to study the developmental and physiological characteristics of a variety of cell types is increasing, due in large part to their ease of integration with tissue engineering, regenerative medicine, and high-throughput screening applications. In this study, myotubes derived from fetal rat hind limbs were induced to develop several aspects of mature muscle including: sarcomere assembly, development of the excitation-contraction coupling apparatus and myosin heavy chain (MHC) class switching. Utilizing immunocytochemical analysis, anisotropic and isotropic band formation (striations) within the myotubes was established, indicative of sarcomere formation. In addition, clusters of ryanodine receptors were colocalized with dihydropyridine complex proteins which signaled development of the excitation-contraction coupling apparatus and transverse tubule biogenesis. The myotubes also exhibited MHC class switching from embryonic to neonatal MHC. Lastly, the myotubes survived significantly longer in culture (70-90 days) than myotubes from our previously developed system (20-25 days). These results were achieved by modifying the culture timeline as well as the development of a new medium formulation. This defined model system for skeletal muscle maturation supports the goal of developing physiologically relevant muscle constructs for use in tissue engineering and regenerative medicine as well as for high-throughput screening applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.