ArticleBMC genomics2009
Genome-wide analysis of the RpoN regulon in Geobacter sulfurreducens.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 60 citations in OpenAlex.
- Influence of support materials on the electroactive behavior, structure and gene expression of wild type and GSU1771-deficient mutant of Geobacter sulfurreducens biofilms.Environmental science and pollution research international · 2025Article
- ProPr54 web server: predicting σNAR genomics and bioinformatics · 2025Article
- Analysis and comparison of the bacterial σ54 regulon: Evidence of phylogenetic trends in gene regulation.PloS one · 2025Article
- Nitrogen Fixation and Ammonium Assimilation Pathway Expression of Geobacter sulfurreducens Changes in Response to the Anode Potential in Microbial Electrochemical Cells.Applied and environmental microbiology · 2023Article
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- Specialization of the Reiterated Copies of the Heterodimeric Integration Host Factor Genes inFrontiers in microbiology · 2021Article
- Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria.Nature communications · 2019Article
- Regulation ofEcoSal Plus · 2017Review
- Genetic switches and related tools for controlling gene expression and electrical outputs of Geobacter sulfurreducens.Journal of industrial microbiology & biotechnology · 2016Article
- An advanced bioinformatics approach for analyzing RNA-seq data reveals sigma H-dependent regulation of competence genes in Listeria monocytogenes.BMC genomics · 2016Article
- Identification of N(α)-acetyl-α-lysine as a probable thermolyte and its accumulation mechanism in Salinicoccus halodurans H3B36.Scientific reports · 2015Article
- σ54-dependent regulome in Desulfovibrio vulgaris Hildenborough.BMC genomics · 2015Article
- Identification of metabolism pathways directly regulated by sigma(54) factor in Bacillus thuringiensis.Frontiers in microbiology · 2015Article
- Use of a promiscuous, constitutively-active bacterial enhancer-binding protein to define the σ⁵⁴ (RpoN) regulon of Salmonella Typhimurium LT2.BMC genomics · 2013Article
- Variation among Desulfovibrio species in electron transfer systems used for syntrophic growth.Journal of bacteriology · 2013Article
- Characterizing the interplay between multiple levels of organization within bacterial sigma factor regulatory networks.Nature communications · 2013Article
- Phylogenetic classification of diverse LysR-type transcriptional regulators of a model prokaryote Geobacter sulfurreducens.Journal of molecular evolution · 2012Article
- Identification of multicomponent histidine-aspartate phosphorelay system controlling flagellar and motility gene expression in Geobacter species.The Journal of biological chemistry · 2012Article
- Identification of a transcriptional repressor involved in benzoate metabolism in Geobacter bemidjiensis.Applied and environmental microbiology · 2011Article
- The zinc-ribbon domain of Helicobacter pylori HP0958: requirement for RpoN accumulation and possible roles of homologs in other bacteria.Microbial informatics and experimentation · 2011Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe role of the RNA polymerase sigma factor RpoN in regulation of gene expression in Geobacter sulfurreducens was investigated to better understand transcriptional regulatory networks as part of an effort to develop regulatory modules for genome-scale in silico models, which can predict the physiological responses of Geobacter species during groundwater bioremediation or electricity production.
resultsAn rpoN deletion mutant could not be obtained under all conditions tested. In order to investigate the regulon of the G. sulfurreducens RpoN, an RpoN over-expression strain was made in which an extra copy of the rpoN gene was under the control of a taclac promoter. Combining both the microarray transcriptome analysis and the computational prediction revealed that the G. sulfurreducens RpoN controls genes involved in a wide range of cellular functions. Most importantly, RpoN controls the expression of the dcuB gene encoding the fumarate/succinate exchanger, which is essential for cell growth with fumarate as the terminal electron acceptor in G. sulfurreducens. RpoN also controls genes, which encode enzymes for both pathways of ammonia assimilation that is predicted to be essential under all growth conditions in G. sulfurreducens. Other genes that were identified as part of the RpoN regulon using either the computational prediction or the microarray transcriptome analysis included genes involved in flagella biosynthesis, pili biosynthesis and genes involved in central metabolism enzymes and cytochromes involved in extracellular electron transfer to Fe(III), which are known to be important for growth in subsurface environment or electricity production in microbial fuel cells. The consensus sequence for the predicted RpoN-regulated promoter elements is TTGGCACGGTTTTTGCT.
conclusionThe G. sulfurreducens RpoN is an essential sigma factor and a global regulator involved in a complex transcriptional network controlling a variety of cellular processes.
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