ArticleBlood2009
Distinct spatio-temporal Ca2+ signaling elicited by integrin alpha2beta1 and glycoprotein VI under flow.
Article in Blood, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Integrin α2: mode of regulation and functioning in the metastatic cancer cascade.Journal of translational medicine · 2026Review
- Smart Clot: An Automated Point-of-Care Flow Assay for Quantitative Whole-Blood Platelet, Fibrin, and Thrombus Kinetics.Biosensors · 2026Article
- Impact of pneumatic tube vs. bicycle courier transport on platelet aggregation: influence of sex and diabetes.Frontiers in medicine · 2025Article
- A New Shear-Stress-Based Point-of-Care Technology for Evaluation of the Hemostatic Pattern in Whole Blood.Biosensors · 2024Article
- Platelet integrin activation surfs the calcium waves.Platelets · 2021Article
- Tspan18 is a novel regulator of thrombo-inflammation.Medical microbiology and immunology · 2020Review
- Tau-Induced CaNeuroscience bulletin · 2018Article
- Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation.Platelets · 2017Article
- Differential integrin activity mediated by platelet collagen receptor engagement under flow conditions.Thrombosis and haemostasis · 2017Article
- Impedance biosensor for real-time monitoring and prediction of thrombotic individual profile in flowing blood.PloS one · 2017Article
- Fibronectin maintains the balance between hemostasis and thrombosis.Cellular and molecular life sciences : CMLS · 2016Review
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- α2β1 integrin, GPVI receptor, and common FcRγ chain on mouse platelets mediate distinct responses to collagen in models of thrombosis.PloS one · 2014Article
- Laminin interactions with head and neck cancer cells under low fluid shear conditions lead to integrin activation and binding.The Journal of biological chemistry · 2012Article
- A role for adhesion and degranulation-promoting adapter protein in collagen-induced platelet activation mediated via integrin α(2) β(1).Journal of thrombosis and haemostasis : JTH · 2012Article
- Emerging diagnostic and therapeutic molecular imaging applications in vascular disease.Vascular medicine (London, England) · 2011Review
- Differential proteomic analysis of platelets suggested possible signal cascades network in platelets treated with salvianolic acid B.PloS one · 2011Article
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
We studied how integrin alpha2beta1 and glycoprotein VI (GPVI) contribute to collagen-induced platelet activation under flow conditions by evaluating stable adhesion and intracellular Ca(2+) concentration ([Ca(2+)](i)) of FLUO 3-AM-labeled platelets perfused over acid-soluble type I or microfibrillar type VI collagen. Adhering platelets displayed 2 kinds of [Ca(2+)](i) oscillations. Rapid alpha-like peaks were unaffected by the membrane-impermeable Ca(2+) chelator ethyleneglycoltetraacetic acid but abolished by membrane-permeable BAPTA-AM. Longer-lasting gamma-like peaks were always preceded by at least one alpha-like peak and abolished by intracellular or extracellular Ca(2+) chelation. Inhibition of phosphatidylinositol 3-kinase or phospholipase C and modulation of cyclic nucleotides, but not blockage of adenosine diphosphate receptors, prevented both Ca(2+) responses. Human or mouse platelets lacking GPVI function exhibited alpha-like but not gamma-like Ca(2+) peaks, whereas those lacking alpha2beta1 showed markedly reduced to absent alpha-like and no gamma-like Ca(2+) peaks. Specific alpha2beta1 ligation induced alpha-like but not gamma-like peaks. Thus, alpha2beta1 may generate Ca(2+) signals that are reinforced by GPVI and required for subsequent longer-lasting Ca(2+) oscillation mediated by GPVI through transmembrane ion flux. Our results delineate a GPVI-independent signaling role of alpha2beta1 in response to collagen stimulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.