ArticleThe Plant cell2009
The histidine kinases CYTOKININ-INDEPENDENT1 and ARABIDOPSIS HISTIDINE KINASE2 and 3 regulate vascular tissue development in Arabidopsis shoots.
Article in The Plant cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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64 citing papers in PubMed, 149 citations in OpenAlex.
- The cytokinin histidine kinase receptors regulate nodulation, shoot and root development inFrontiers in plant science · 2026Article
- An updated history of TDIF-PXY signalling: a study in cell fate and tissue patterning.The New phytologist · 2025Review
- Non-Canonical Inter-Protein Interactions of Key Proteins Belonging to Cytokinin Signaling Pathways.Plants (Basel, Switzerland) · 2025Review
- Genome-wide characterization of two-component system elements in barley enables the identification of grain-specific phosphorelay genes.BMC plant biology · 2025Article
- Genome-wide identification and functional characterization of the two-component system gene family in petunia reveals roles in hormone signaling and stress response.Frontiers in plant science · 2025Article
- Primary multistep phosphorelay activation comprises both cytokinin and abiotic stress responses: insights from comparative analysis of Brassica type-A response regulators.Journal of experimental botany · 2024Article
- Genome-wide identification and expression analysis of two-component system genes in switchgrass (Panicum virgatum L.).BMC plant biology · 2024Article
- Cytokinin: From autoclaved DNA to two-component signaling.The Plant cell · 2024Review
- Transcriptome profiling reveals the genes involved in tuberous root expansion in Pueraria (Pueraria montana var. thomsonii).BMC plant biology · 2023Article
- The mutation of CaCKI1 causes seedless fruits in chili pepper (Capsicum annuum).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Transcriptomic analysis of tuberous root in two sweet potato varieties reveals the important genes and regulatory pathways in tuberous root development.BMC genomics · 2022Article
- MusaATAF2-like protein regulates shoot development and multiplication by inducing cytokinin hypersensitivity and flavonoid accumulation in banana plants.Plant cell reports · 2022Article
- Article
- Genome-wide identification and expression analysis of two-component system genes in sweet potato (Frontiers in plant science · 2022Article
- Identification and Characterization of Key Genes Responsible for Weedy and Cultivar Growth Types in Soybean.Frontiers in genetics · 2022Article
- Article
- Signal Integration in Plant Abiotic Stress Responses via Multistep Phosphorelay Signaling.Frontiers in plant science · 2021Review
- Mutagenomics: A Rapid, High-Throughput Method to Identify Causative Mutations from a Genetic Screen.Plant physiology · 2020Article
- The Importance of Cytokinins during Reproductive Development in Arabidopsis and Beyond.International journal of molecular sciences · 2020Review
- Differences in xylem development between Dutch and Japanese tomato (Solanum lycopersicum) correlate with cytokinin levels in hypocotyls.Annals of botany · 2020Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development and activity of the procambium and cambium, which ensure vascular tissue formation, is critical for overall plant architecture and growth. However, little is known about the molecular factors affecting the activity of vascular meristems and vascular tissue formation. Here, we show that the His kinase CYTOKININ-INDEPENDENT1 (CKI1) and the cytokinin receptors ARABIDOPSIS HISTIDINE KINASE2 (AHK2) and AHK3 are important regulators of vascular tissue development in Arabidopsis thaliana shoots. Genetic modifications of CKI1 activity in Arabidopsis cause dysfunction of the two-component signaling pathway and defects in procambial cell maintenance. CKI1 overexpression in protoplasts leads to cytokinin-independent activation of the two-component phosphorelay, and intracellular domains are responsible for the cytokinin-independent activity of CKI1. CKI1 expression is observed in vascular tissues of inflorescence stems, and CKI1 forms homodimers both in vitro and in planta. Loss-of-function ahk2 and ahk3 mutants and plants with reduced levels of endogenous cytokinins show defects in procambium proliferation and an absence of secondary growth. CKI1 overexpression partially rescues ahk2 ahk3 phenotypes in vascular tissue, while the negative mutation CKI1H405Q further accentuates mutant phenotypes. These results indicate that the cytokinin-independent activity of CKI1 and cytokinin-induced AHK2 and AHK3 are important for vascular bundle formation in Arabidopsis.
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