ArticleJournal of biotechnology2009
Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells.
Article in Journal of biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 137 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
137 citing papers in PubMed, 282 citations in OpenAlex.
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- Applications of Metabolomics to the Clinical Management of Breast Cancer: New Perspectives for Diagnosis, Treatment and Prognosis.International journal of molecular sciences · 2026Review
- Mass Spectrometry as a Tool for Metabolomics in Cancer.Advances in experimental medicine and biology · 2026Review
- Investigating Overflow Metabolism in Heterotrophic Cultures of the Green AlgabioRxiv : the preprint server for biology · 2025Article
- Branched-chain amino acids contribute to diabetic kidney disease progression via PKM2-mediated podocyte metabolic reprogramming and apoptosis.Nature communications · 2025Article
- Simultaneous in vivo multi-organ fluxomics reveals divergent metabolic adaptations in liver, heart, and skeletal muscle during obesity.Cell reports · 2025Article
- Surface-Dependent Isotopic Adsorption of CO on α-AlMolecules (Basel, Switzerland) · 2025Article
- An organism-level quantitative flux model of energy metabolism in mice.Cell metabolism · 2025Article
- Analyses of mitochondrial metabolism in diseases: a review onRSC advances · 2024Review
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- Direct Infusion Mass Spectrometry to Rapidly Map Metabolic Flux of Substrates Labeled with Stable Isotopes.Metabolites · 2024Article
- Oleic acid differentially affects lipid droplet storage ofChemical communications (Cambridge, England) · 2024Article
- The Warburg Effect Reinterpreted 100 yr on: A First-Principles Stoichiometric Analysis and Interpretation from the Perspective of ATP Metabolism in Cancer Cells.Function (Oxford, England) · 2024Article
- Metabolic flux analysis in adipose tissue reprogramming.Immunometabolism (Cobham, Surrey) · 2024Review
- Oleic acid differentially affectsbioRxiv : the preprint server for biology · 2023Article
- The pentose phosphate pathway in health and disease.Nature metabolism · 2023Review
- Monitoring and modelling the glutamine metabolic pathway: a review and future perspectives.Metabolomics : Official journal of the Metabolomic Society · 2023Review
77 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
(13)C metabolic flux analysis (MFA) is the most comprehensive means of characterizing cellular metabolic states. Uniquely labeled isotopic tracers enable more focused analyses to probe specific reactions within the network. As a result, the choice of tracer largely determines the precision with which one can estimate metabolic fluxes, especially in complex mammalian systems that require multiple substrates. Here we have experimentally determined metabolic fluxes in a tumor cell line, successfully recapitulating the hallmarks of cancer cell metabolism. Using these data, we computationally evaluated specifically labeled (13)C glucose and glutamine tracers for their ability to precisely and accurately estimate fluxes in central carbon metabolism. These methods enabled us to identify the optimal tracer for analyzing individual fluxes, specific pathways, and central carbon metabolism as a whole. [1,2-(13)C(2)]glucose provided the most precise estimates for glycolysis, the pentose phosphate pathway, and the overall network. Tracers such as [2-(13)C]glucose and [3-(13)C]glucose also outperformed the more commonly used [1-(13)C]glucose. [U-(13)C(5)]glutamine emerged as the preferred isotopic tracer for the analysis of the tricarboxylic acid (TCA) cycle. These results provide valuable, quantitative information on the performance of (13)C-labeled substrates and can aid in the design of more informative MFA experiments in mammalian cell culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.