ArticleBiochemistry2009
Transmembrane topology of the mammalian Slc11a2 iron transporter.
Article in Biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Functional Characterization of the P-Type ATPases ATP13A3 and ATP13A4 as Plasma Membrane Polyamine Transporters.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Identification of a divalent metal transporter required for cellular iron metabolism in malaria parasites.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Identification of a divalent metal transporter required for cellular iron metabolism in malaria parasites.bioRxiv : the preprint server for biology · 2024Article
- Biocompatible Iron Oxide Nanoparticles Display Antiviral Activity Against Two Different Respiratory Viruses in Mice.International journal of nanomedicine · 2024Article
- Iron derived from NCOA4-mediated ferritinophagy causes cellular senescence via the cGAS-STING pathway.Cell death discovery · 2023Article
- Iron oxide and iron oxyhydroxide nanoparticles impair SARS-CoV-2 infection of cultured cells.Journal of nanobiotechnology · 2022Article
- Molecular Mechanism of Nramp-Family Transition Metal Transport.Journal of molecular biology · 2021Review
- COVID-19, ferrosenescence and neurodegeneration, a mini-review.Progress in neuro-psychopharmacology & biological psychiatry · 2021Review
- Transmembrane protein western blotting: Impact of sample preparation on detection of SLC11A2 (DMT1) and SLC40A1 (ferroportin).PloS one · 2020Article
- Article
- Mutation at Different Sites of Metal Transporter GeneFrontiers in plant science · 2019Article
- MntC-Dependent Manganese Transport Is Essential formSphere · 2018Article
- Targeting iron metabolism in drug discovery and delivery.Nature reviews. Drug discovery · 2017Review
- Regulation of divalent metal transporter-1 by serine phosphorylation.The Biochemical journal · 2016Article
- In vitro permissiveness of bovine neutrophils and monocyte derived macrophages to Leishmania donovani of Ethiopian isolate.Parasites & vectors · 2016Article
- In silico analysis of consequences of non-synonymous SNPs of Slc11a2 gene in Indian bovines.Genomics data · 2015Article
- Dictyostelium Nramp1, which is structurally and functionally similar to mammalian DMT1 transporter, mediates phagosomal iron efflux.Journal of cell science · 2015Article
- Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport.Nature structural & molecular biology · 2014Article
- Transport of magnesium by a bacterial Nramp-related gene.PLoS genetics · 2014Article
- Planar cell polarity pathway regulates nephrin endocytosis in developing podocytes.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The mammalian Slc11a1 and Slc11a2 proteins define a large family of secondary metal transporters. Slc11a1 and Slc11a2 function as pH-dependent divalent cation transporters that play a critical role in host defenses against infections and in Fe2+ homeostasis, respectively. The position and polarity of individual transmembrane domains (TMD) of Slc11a2 were studied by an epitope tagging method based on the insertion of small antigenic hemagglutinin A (HA) peptides (YPYDVPDYAS) in predicted intra- or extracellular loops of the protein. The tagged proteins were expressed in transfected LLC-PK1 kidney cells and tested for transport activity, and the polarity of inserted tags with respect to the plasma membrane was determined by immunofluorescence in intact and permeabilized cells. HA epitope tags were inserted at positions 1, 98, 131, 175, 201, 243, 284, 344, 403, 432, 468, 504, and 561. Insertions at positions 98, 131, 175, 403, and 432 abrogated metal transport by Slc11a2, while insertions at positions 1, 201, 243, 284, 344, 468, 504, and 561 resulted in functional proteins. Topology mapping in functional HA-tagged Slc11a2 proteins indicated that the N-terminus (1), as well as loops delineated by TMD4-5 (201), TMD6-7 (284), and TMD10-11 (468), and C-terminus (561) are intracellular, while loops separating TMD5-6 (243), TMD7-8 (344), and TMD11-12 (504) are extracellular. These results are compatible with a topology of 12 transmembrane domains, with intracellular amino and carboxy termini. Structural models constructed by homology threading support this 12TMD topology and show 2-fold structural symmetry in the arrangement of membrane helices for TM1-5 and TM6-10 (conserved Slc11 hydrophobic core).
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.