ArticlePLoS biology2009
The neuropeptide PDF acts directly on evening pacemaker neurons to regulate multiple features of circadian behavior.
Article in PLoS biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed.
- Splicing of a core circadian clock gene regulates seasonal adaptations by a winter gating mechanism.Science advances · 2026Article
- Soma CaCurrent biology : CB · 2026Article
- Sleep regulation inFrontiers in neuroscience · 2026Review
- Remote and partial clocks expand the circadian neuronal network, driving widespread molecular rhythmicity inbioRxiv : the preprint server for biology · 2025Article
- Splicing of a core clock gene regulates seasonal adaptations by a winter gating mechanism.bioRxiv : the preprint server for biology · 2025Article
- Neuropeptide dynamics coordinate layered plasticity mechanisms adaptingScience advances · 2025Article
- Mutual coupling of neurons in the circadian master clock: What we can learn from fruit flies.Neurobiology of sleep and circadian rhythms · 2025Article
- Glial Regulation of Circuit Wiring, Firing, and Expiring in theCold Spring Harbor perspectives in biology · 2024Review
- Article
- Integration of photoperiodic and temperature cues by the circadian clock to regulate insect seasonal adaptations.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2024Review
- Autophagy as a new player in the regulation of clock neurons physiology of Drosophila melanogaster.Scientific reports · 2024Article
- Molecular and Neural Mechanisms of Temperature Preference Rhythm inJournal of biological rhythms · 2023Review
- Restoration of Sleep and Circadian Behavior by Autophagy Modulation in Huntington's Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Allnighter pseudokinase-mediated feedback links proteostasis and sleep in Drosophila.Nature communications · 2023Article
- Seasonal cues act through the circadian clock and pigment-dispersing factor to control EYES ABSENT and downstream physiological changes.Current biology : CB · 2023Article
- Modulation of Metabolic Hormone Signaling via a Circadian Hormone and Biogenic Amine inInternational journal of molecular sciences · 2022Article
- Article
- The Neuropeptide PDF Is Crucial for Delaying the Phase ofJournal of biological rhythms · 2021Article
- Integration of Circadian Clock Information in theJournal of biological rhythms · 2021Review
- Daily rewiring of a neural circuit generates a predictive model of environmental light.Science advances · 2021Article
8 more citing papers are in PubMed but not listed here.
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3 authors.
Funding
Abstract
Discrete clusters of circadian clock neurons temporally organize daily behaviors such as sleep and wake. In Drosophila, a network of just 150 neurons drives two peaks of timed activity in the morning and evening. A subset of these neurons expresses the neuropeptide pigment dispersing factor (PDF), which is important for promoting morning behavior as well as maintaining robust free-running rhythmicity in constant conditions. Yet, how PDF acts on downstream circuits to mediate rhythmic behavior is unknown. Using circuit-directed rescue of PDF receptor mutants, we show that PDF targeting of just approximately 30 non-PDF evening circadian neurons is sufficient to drive morning behavior. This function is not accompanied by large changes in core molecular oscillators in light-dark, indicating that PDF RECEPTOR likely regulates the output of these cells under these conditions. We find that PDF also acts on this focused set of non-PDF neurons to regulate both evening activity phase and period length, consistent with modest resetting effects on core oscillators. PDF likely acts on more distributed pacemaker neuron targets, including the PDF neurons themselves, to regulate rhythmic strength. Here we reveal defining features of the circuit-diagram for PDF peptide function in circadian behavior, revealing the direct neuronal targets of PDF as well as its behavioral functions at those sites. These studies define a key direct output circuit sufficient for multiple PDF dependent behaviors.
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