ReviewNature chemical biology2009
Directed evolution drives the next generation of biocatalysts.
Review in Nature chemical biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 192 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
192 citing papers in PubMed.
- Article
- Continuous Evolution System Based on Toxin-Antitoxin System Combined with Base Deaminase Accelerates the In Vivo Modification of Target Proteins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Genuine Directed Evolution In Test Tube (GENie).bioRxiv : the preprint server for biology · 2026Article
- Creating highly active fluoroacetate dehalogenases via gate-based synergetic chain design.Nature communications · 2026Article
- MechFind: a computational framework for de novo prediction of enzyme mechanisms.Nature communications · 2026Article
- Accelerated discovery of highly active enzyme nanohybrids with parallelized Bayesian optimization in hybrid space.Nature communications · 2026Article
- Tuning aromatic cage occupancy in prenyltransferases enables selective and efficient production of rare c-prenylated flavonoids.Nature communications · 2026Article
- Efficient, complete biosynthesis of isoflavonoid calycosin-7-glucoside by metabolically engineered yeast cell factories.Frontiers in bioengineering and biotechnology · 2026Article
- Directed evolution of a TNA polymerase identifies independent paths to fidelity and catalysis.Nature communications · 2025Article
- Phenotyping and Selection of Cells Using Mass Spectrometry and a Microfluidic Droplet Printer.Analytical chemistry · 2025Article
- MechFind: A computational framework for de novo prediction of enzyme mechanisms.bioRxiv : the preprint server for biology · 2025Article
- Engineering protein prenylation: an emerging tool for selective protein modification.Biochemical Society transactions · 2025Review
- Tech-Enhanced Synthesis: Exploring the Synergy between Organic Chemistry and Technology.Journal of the American Chemical Society · 2025Review
- Article
- Engineering 2-Deoxy-D-ribose-5-phosphate Aldolase for anti- and syn-Selective Epoxidations of α,β-Unsaturated Aldehydes.Angewandte Chemie (International ed. in English) · 2025Article
- Navigating Side Reactions for Robust Colorimetric Detection of Galactose Oxidase Activity.Analytical chemistry · 2025Article
- Covalent Template-Directed Synthesis: A Powerful Tool for the Construction of Complex Molecules.Chemical reviews · 2025Review
- Bioengineering hybrid artificial life.Frontiers in bioinformatics · 2025Review
- A Quantum Mechanical Approach to The Mechanism of Asymmetric Synthesis of Chiral Amine by Imine Reductase from Stackebrandtia Nassauensis.ChemPlusChem · 2025Article
- Radical-Mediated Regiodivergent C(Chem catalysis · 2024Article
132 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzymes are increasingly being used as biocatalysts in the generation of products that have until now been derived using traditional chemical processes. Such products range from pharmaceutical and agrochemical building blocks to fine and bulk chemicals and, more recently, components of biofuels. For a biocatalyst to be effective in an industrial process, it must be subjected to improvement and optimization, and in this respect the directed evolution of enzymes has emerged as a powerful enabling technology. Directed evolution involves repeated rounds of (i) random gene library generation, (ii) expression of genes in a suitable host and (iii) screening of libraries of variant enzymes for the property of interest. Both in vitro screening-based methods and in vivo selection-based methods have been applied to the evolution of enzyme function and properties. Significant developments have occurred recently, particularly with respect to library design, screening methodology, applications in synthetic transformations and strategies for the generation of new enzyme function.
Indexed as
Identifiers
19620998What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.