ArticleProceedings of the National Academy of Sciences of the United States of America2009
Protein targeting into secondary plastids of chlorarachniophytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Recent plastid replacement in Karlodinium ballantinum (Kareniaceae, Dinoflagellata) challenges the paradigms of endosymbiotic gene transfer.Molecular biology and evolution · 2026Article
- Nuclear genome sequencing reveals the highly intron-rich architecture of the chlorarachniophyte alga Amorphochlora amoebiformis.DNA research : an international journal for rapid publication of reports on genes and genomes · 2025Article
- Pyrenoid proteomics reveals independent evolution of the COProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Encyclopedia of Family A DNA Polymerases Localized in Organelles: Evolutionary Contribution of Bacteria Including the Proto-Mitochondrion.Molecular biology and evolution · 2024Article
- Dinoflagellates with relic endosymbiont nuclei as models for elucidating organellogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Verification of theInternational journal of molecular sciences · 2019Article
- Organellar DNA Polymerases in Complex Plastid-Bearing Algae.Biomolecules · 2019Article
- Extensive Reduction of the Nuclear Pore Complex in Nucleomorphs.Genome biology and evolution · 2019Article
- Diurnal Transcriptional Regulation of Endosymbiotically Derived Genes in the Chlorarachniophyte Bigelowiella natans.Genome biology and evolution · 2016Article
- Prospective function of FtsZ proteins in the secondary plastid of chlorarachniophyte algae.BMC plant biology · 2015Article
- Article
- Genome-based reconstruction of the protein import machinery in the secondary plastid of a chlorarachniophyte alga.Eukaryotic cell · 2012Article
- Proteomics reveals plastid- and periplastid-targeted proteins in the chlorarachniophyte alga Bigelowiella natans.Genome biology and evolution · 2012Article
- Tetrapyrrole synthesis of photosynthetic chromerids is likely homologous to the unusual pathway of apicomplexan parasites.The Plant cell · 2011Article
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most plastid proteins are encoded by the nuclear genome, and consequently, need to be transported into plastids across multiple envelope membranes. In diverse organisms possessing secondary plastids, nuclear-encoded plastid precursor proteins (preproteins) commonly have an N-terminal extension that consists of an endoplasmic reticulum (ER)-targeting signal peptide and a transit peptide-like sequence (TPL). This bipartite targeting peptide is believed to be necessary for targeting the preproteins into the secondary plastids. Here, we newly demonstrate the function of the bipartite targeting peptides of an algal group, chlorarachniophytes, and characterize the functional domains of the TPL in the precursor of a plastid protein, ATP synthase delta subunit (AtpD), using a GFP as a reporter molecule. We show that the C-terminal portion of the TPL is important for targeting the AtpD preprotein from the ER into the chlorarachniophyte plastids, and several positively charged amino acids in the TPL are also necessary for transporting the preprotein across the 2 innermost plastid membranes. Compared with other groups with secondary plastids, the TPL functional domains of the chlorarachniophytes are unique, which might be caused by independent acquisition of their plastids.
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