ArticleThe Journal of cell biology2009
Membrane proteins follow multiple pathways to the basolateral cell surface in polarized epithelial cells.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 89 citations in OpenAlex.
- Biotin methyl ester enhances cargo release in RUSH system and enables rapid biotinylation with TurboID.Communications biology · 2025Article
- Studying Cargo Transport Using RudLOV.Bio-protocol · 2025Article
- Polarized transport of membrane and secreted proteins during lumen morphogenesis.Seminars in cell & developmental biology · 2023Review
- Identification of Cellular Genes Involved in Baculovirus GP64 Trafficking to the Plasma Membrane.Journal of virology · 2022Article
- Kidney epithelial cells are active mechano-biological fluid pumps.Nature communications · 2022Article
- Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells.Current biology : CB · 2022Article
- PolarProtPred: predicting apical and basolateral localization of transmembrane proteins using putative short linear motifs and deep learning.Bioinformatics (Oxford, England) · 2021Article
- Myosin II isoforms promote internalization of spatially distinct clathrin-independent endocytosis cargoes through modulation of cortical tension downstream of ROCK2.Molecular biology of the cell · 2021Article
- Newly synthesized polycystin-1 takes different trafficking pathways to the apical and ciliary membranes.Traffic (Copenhagen, Denmark) · 2018Article
- New approaches for solving old problems in neuronal protein trafficking.Molecular and cellular neurosciences · 2018Review
- Basolateral delivery of the type I transforming growth factor beta receptor is mediated by a dominant-acting cytoplasmic motif.Molecular biology of the cell · 2017Article
- Golgi-independent secretory trafficking through recycling endosomes in neuronal dendrites and spines.eLife · 2017Article
- The secretory pathway at 50: a golden anniversary for some momentous grains of silver.Molecular biology of the cell · 2017Article
- Newly synthesized and recycling pools of the apical protein gp135 do not occupy the same compartments.Traffic (Copenhagen, Denmark) · 2016Article
- A 3-D cell culture system to study epithelia functions using microcarriers.Cytotechnology · 2016Article
- Techniques for studying protein trafficking and molecular motors in neurons.Cytoskeleton (Hoboken, N.J.) · 2016Review
- Dual pulse-chase microscopy reveals early divergence in the biosynthetic trafficking of the Na,K-ATPase and E-cadherin.Molecular biology of the cell · 2015Article
- The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135.The Journal of cell biology · 2015Article
- Kv1.3 contains an alternative C-terminal ER exit motif and is recruited into COPII vesicles by Sec24a.BMC biochemistry · 2015Article
- Endogenously tagged rab proteins: a resource to study membrane trafficking in Drosophila.Developmental cell · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Newly synthesized apical and basolateral membrane proteins are sorted from one another in polarized epithelial cells. The trans-Golgi network participates in this sorting process, but some basolateral proteins travel from the Golgi to recycling endosomes (REs) before their surface delivery. Using a novel system for pulse-chase microscopy, we have visualized the postsynthetic route pursued by a newly synthesized cohort of Na,K-ATPase. We find that the basolateral delivery of newly synthesized Na,K-ATPase occurs via a pathway distinct from that pursued by the vesicular stomatitis virus G protein (VSV-G). Na,K-ATPase surface delivery occurs at a faster rate than that observed for VSV-G. The Na,K-ATPase does not pass through the RE compartment en route to the plasma membrane, and Na,K-ATPase trafficking is not regulated by the same small GTPases as other basolateral proteins. Finally, Na,K-ATPase and VSV-G travel in separate post-Golgi transport intermediates, demonstrating directly that multiple routes exist for transport from the Golgi to the basolateral membrane in polarized epithelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.