ArticleThe Journal of biological chemistry2009
Directionality and coordination of dehydration and ring formation during biosynthesis of the lantibiotic nisin.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Structural insights into the substrate binding mechanism of the class I dehydratase MadB.Communications biology · 2025Article
- Insights into the production and evolution of lantibiotics from a computational analysis of peptides associated with the lanthipeptide cyclase domain.Royal Society open science · 2024Article
- Phylogeny-guided genome mining of roseocin family lantibiotics to generate improved variants of roseocin.AMB Express · 2023Article
- Article
- Evaluation of Apoptotic Gene Expression in Hepatoma Cell Line (HepG2) Following Nisin Treatment.Asian Pacific journal of cancer prevention : APJCP · 2021Article
- Article
- Impact of the nisin modification machinery on the transport kinetics of NisT.Scientific reports · 2020Article
- A Structural View on the Maturation of Lanthipeptides.Frontiers in microbiology · 2020Review
- Insights in the Antimicrobial Potential of the Natural Nisin Variant Nisin H.Frontiers in microbiology · 2020Article
- Characterization of glutamyl-tRNA-dependent dehydratases using nonreactive substrate mimics.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Systematic characterization of position one variants within the lantibiotic nisin.Scientific reports · 2019Article
- Bioprocess Development for Lantibiotic Ruminococcin-A Production inFrontiers in microbiology · 2019Article
- Feasability of Introducing a Thioether Ring in Vasopressin byFrontiers in microbiology · 2019Article
- Modifying the Lantibiotic Mutacin 1140 for Increased Yield, Activity, and Stability.Applied and environmental microbiology · 2018Article
- Increasing the Antimicrobial Activity of Nisin-Based Lantibiotics against Gram-Negative Pathogens.Applied and environmental microbiology · 2018Article
- Characterization of Leader Peptide Binding During Catalysis by the Nisin Dehydratase NisB.Journal of the American Chemical Society · 2018Article
- Specificity and Application of the Lantibiotic Protease NisP.Frontiers in microbiology · 2018Article
- Heterologous Biosynthesis, Modifications and Structural Characterization of Ruminococcin-A, a Lanthipeptide From the Gut BacteriumFrontiers in microbiology · 2018Article
- Phage display and selection of lanthipeptides on the carboxy-terminus of the gene-3 minor coat protein.Nature communications · 2017Article
- Recombinant Macrocyclic Lanthipeptides Incorporating Non-Canonical Amino Acids.Journal of the American Chemical Society · 2017Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lantibiotic nisin is a potent antimicrobial substance, which contains unusual lanthionine rings and dehydrated amino acid residues and is produced by Lactococcus lactis. Recently, the nisin biosynthetic machinery has been applied to introduce lanthionine rings in peptides other than nisin with potential therapeutic use. Due to difficulties in the isolation of the proposed synthetase complex (NisBTC), mechanistic information concerning the enzymatic biosynthesis of nisin is scarce. Here, we present the molecular characterization of a number of nisin mutants that affect ring formation. We have investigated in a systematic manner how these mutations influence dehydration events, which are performed enzymatically by the dehydratase NisB. Specific mutations that hampered ring formation allowed for the dehydration of serine residues that directly follow the rings and are normally unmodified. The combined information leads to the conclusion that 1) nisin biosynthesis is an organized directional process that starts at the N terminus of the molecule and continues toward the C terminus, and 2) NisB and NisC are alternating enzymes, whose activities follow one after another in a repetitive way. Thus, the dehydration and cyclization processes are not separated in time and space. On the basis of these results and previous knowledge, a working model for the sequence of events in the maturation of nisin is proposed.
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