ArticleRNA (New York, N.Y.)2009
Quantitative analysis of zeptomole microRNAs based on isothermal ramification amplification.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Emerging Biomarkers and Nanobiosensing Strategies in Diabetes.Biosensors · 2025Review
- In Silico Prediction and Molecular Docking of SNPs in NRP1 Gene Associated with SARS-COV-2.Biochemical genetics · 2024Article
- Multiplexed miRNA and Protein Analysis Using Digital Quantitative PCR in Microwell Arrays.Analytical chemistry · 2024Article
- Single-Walled Carbon Nanotube Sensor Selection for the Detection of MicroRNA Biomarkers for Acute Myocardial Infarction as a Case Study.ACS sensors · 2023Article
- Isothermal nucleic acid amplification and its uses in modern diagnostic technologies.3 Biotech · 2023Review
- A Combinational Approach for More Efficient miRNA Biosensing.Current genomics · 2022Review
- Physical and chemical template-blocking strategies in the exponential amplification reaction of circulating microRNAs.Analytical and bioanalytical chemistry · 2020Article
- Quantification of microRNAs directly from body fluids using a base-stacking isothermal amplification method in a point-of-care device.Biomedical microdevices · 2017Article
- Differential TGFβ pathway targeting by miR-122 in humans and mice affects liver cancer metastasis.Nature communications · 2016Article
- Quantification of mature microRNAs using pincer probes and real-time PCR amplification.PloS one · 2015Article
- Sensitive PCR-based quantitation of cell-free circulating microRNAs.Methods (San Diego, Calif.) · 2012Article
- Current approaches to micro-RNA analysis and target gene prediction.Journal of applied genetics · 2012Review
- Genome-wide functional screening of miR-23b as a pleiotropic modulator suppressing cancer metastasis.Nature communications · 2011Article
- Ultrasensitive multiplexed microRNA quantification on encoded gel microparticles using rolling circle amplification.Analytical chemistry · 2011Article
- Point-of-care nucleic acid testing for infectious diseases.Trends in biotechnology · 2011Review
- miR-ID: a novel, circularization-based platform for detection of microRNAs.RNA (New York, N.Y.) · 2011Article
- A mechanism for ramified rolling circle amplification.BMC molecular biology · 2010Article
- High-performance quantification of mature microRNAs by real-time RT-PCR using deoxyuridine-incorporated oligonucleotides and hemi-nested primers.RNA (New York, N.Y.) · 2010Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To date, approximately 700 microRNA (miRNA) molecules have been identified in humans. Accurate and sensitive quantification of miRNA levels will help unveil their biological functions. Here, we extend the isothermal ramification amplification (RAM) approach to a sensitive and specific real-time assay for quantitative analysis of miRNA. This RAM miRNA assay is based on the threshold cycle (C(T)) principle similar to that of real-time PCR. It has a dynamic range of at least seven orders of magnitude, allowing for the quantification of miRNA input from 10(3) to 10(10) copies per reaction (10 nM to 1 fM). The capabilities of discriminating single-base mismatch and distinguishing mature miRNAs from their precursors are achieved by coupling the reverse-transcription of miRNA to the generation of a closed C-probe, rather than using expensive detection probes like in real-time PCR. Quantitative measurement of 5 miRNAs (mir-1, miR-122, mir-150, mir-143, and let-7a) across 12 mouse tissues is validated in total RNA samples without further purification. U6 snRNA, snoRNA 135, and miRNA-191 could be simultaneously quantified as endogenous controls. These results suggest that our RAM miRNA assay might provide a universal tool for miRNA detection and functional studies to meet the needs for bench examination, clinical diagnosis, and on-site detection.
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Registered trials
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