ArticleJournal of molecular biology2009
Multiple metal-binding cores are required for metalloregulation by M-box riboswitch RNAs.
Article in Journal of molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Riboswitch Mechanisms: New Tricks for an Old Dog.Biochemistry. Biokhimiia · 2021Review
- Cooperativity and Allostery in RNA Systems.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Local-to-global signal transduction at the core of a MnNature communications · 2019Article
- A Mn-sensing riboswitch activates expression of a Mn2+/Ca2+ ATPase transporter in Streptococcus.Nucleic acids research · 2019Article
- Metalloriboswitches: RNA-based inorganic ion sensors that regulate genes.The Journal of biological chemistry · 2017Review
- Long-Range Interactions in Riboswitch Control of Gene Expression.Annual review of biophysics · 2017Review
- Characterization of MgACS omega · 2016Article
- Force Field for Mg(2+), Mn(2+), Zn(2+), and Cd(2+) Ions That Have Balanced Interactions with Nucleic Acids.The journal of physical chemistry. B · 2015Article
- Review
- The ubiquitous yybP-ykoY riboswitch is a manganese-responsive regulatory element.Molecular cell · 2015Article
- Bacterial riboswitches cooperatively bind Ni(2+) or Co(2+) ions and control expression of heavy metal transporters.Molecular cell · 2015Article
- Transport of magnesium by a bacterial Nramp-related gene.PLoS genetics · 2014Article
- ArtR, a novel sRNA of Staphylococcus aureus, regulates α-toxin expression by targeting the 5' UTR of sarT mRNA.Medical microbiology and immunology · 2014Article
- Metabolite recognition principles and molecular mechanisms underlying riboswitch function.Annual review of biophysics · 2012Review
- Thermodynamically stable RNA three-way junction for constructing multifunctional nanoparticles for delivery of therapeutics.Nature nanotechnology · 2011Article
- Evidence for a thermodynamically distinct Mg2+ ion associated with formation of an RNA tertiary structure.Journal of the American Chemical Society · 2011Article
- Insights into metalloregulation by M-box riboswitch RNAs via structural analysis of manganese-bound complexes.Journal of molecular biology · 2011Article
- The roles of metal ions in regulation by riboswitches.Metal ions in life sciences · 2011Review
- Metal ions: supporting actors in the playbook of small ribozymes.Metal ions in life sciences · 2011Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Riboswitches are regulatory RNAs that control downstream gene expression in response to direct association with intracellular metabolites or metals. Typically, riboswitch aptamer domains bind to a single small-molecule metabolite. In contrast, an X-ray crystallographic structural model for the M-box riboswitch aptamer revealed the absence of an organic metabolite ligand but the presence of at least six tightly associated magnesiums. This observation agrees well with the proposed role of the M-box riboswitch in functioning as a sensor of intracellular magnesium, although additional nonspecific metal interactions are also undoubtedly required for these purposes. To gain greater functional insight into the metalloregulatory capabilities of M-box RNAs, we sought to determine whether all or a subset of the RNA-chelated magnesium ions were required for riboswitch function. To accomplish this task, each magnesium-binding site was simultaneously yet individually perturbed through random incorporation of phosphorothioate nucleotide analogues, and RNA molecules were investigated for their ability to fold in varying levels of magnesium. These data revealed that all of the magnesium ions observed in the structural model are important for magnesium-dependent tertiary structure formation. Additionally, these functional data revealed a new core of potential metal-binding sites that are likely to assist formation of key tertiary interactions and were previously unobserved in the structural model. It is clear from these data that M-box RNAs require specific binding of a network of metal ions for partial fulfillment of their metalloregulatory functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.