ArticleBiophysical journal2009
Imaging barriers to diffusion by pair correlation functions.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed, 151 citations in OpenAlex.
- Mapping Protein Diffusion from the Plasma Membrane to the Nucleus: Insights from Fluorescence Correlation Spectroscopy.Biomolecules · 2026Review
- Measuring morphogen transport over multiple spatial scales in live zebrafish embryos.Nature communications · 2026Article
- Spatial triple-correlation spectroscopy reveals heterotrimer dynamics in live cells.Biophysical journal · 2026Article
- Spectral demixing enables reliable dual color pair correlation function analysis of viral and cellular proteins in live cells.Communications biology · 2026Article
- D‑Blur: A Deep Learning Approach for Mapping Subdiffraction Diffusion with Motion-Blurred Images.Chemical & biomedical imaging · 2025Article
- Adherent cells sustain membrane tension gradients independently of migration.Nature communications · 2025Article
- Measuring PARP1 mobility at DNA damage sites by segmented fluorescence correlation spectroscopy.Biophysical journal · 2025Article
- Crossover patterning through condensation and coarsening of pro-crossover factors.Nature cell biology · 2025Article
- Pair correlation microscopy of intracellular molecular transport.Nature protocols · 2025Review
- Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope.Scientific reports · 2024Article
- Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications.Communications biology · 2023Review
- Actin filaments form a size-dependent diffusion barrier around centrosomes.EMBO reports · 2023Article
- Dynamic Mode Decomposition of Fluorescence Loss in Photobleaching Microscopy Data for Model-Free Analysis of Protein Transport and Aggregation in Living Cells.Sensors (Basel, Switzerland) · 2022Article
- Radial pair correlation of molecular brightness fluctuations maps protein diffusion as a function of oligomeric state within live-cell nuclear architecture.Biophysical journal · 2022Article
- Heterochromatic repeat clustering imposes a physical barrier on homologous recombination to prevent chromosomal translocations.Molecular cell · 2022Article
- Transbilayer Coupling of Lipids in Cells Investigated by Imaging Fluorescence Correlation Spectroscopy.The journal of physical chemistry. B · 2022Article
- Concepts to Reveal Parvovirus-Nucleus Interactions.Viruses · 2021Review
- Confocal-based fluorescence fluctuation spectroscopy with a SPAD array detector.Light, science & applications · 2021Article
- Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host.Viruses · 2021Review
- New Methodologies to Study DNA Repair Processes in Space and Time Within Living Cells.Frontiers in cell and developmental biology · 2021Review
26 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Molecular diffusion and transport are fundamental processes in physical, chemical, biochemical, and biological systems. However, current approaches to measure molecular transport in cells and tissues based on perturbation methods such as fluorescence recovery after photobleaching are invasive, fluctuation correlation methods are local, and single-particle tracking requires the observation of isolated particles for relatively long periods of time. We propose to detect molecular transport by measuring the time cross-correlation of fluctuations at a pair of locations in the sample. When the points are farther apart than two times the size of the point spread function, the maximum of the correlation is proportional to the average time a molecule takes to move from a specific location to another. We demonstrate the method by simulations, using beads in solution, and by measuring the diffusion of molecules in cellular membranes. The spatial pair cross-correlation method detects barriers to diffusion and heterogeneity of diffusion because the time of the correlation maximum is delayed in the presence of diffusion barriers. This noninvasive, sensitive technique follows the same molecule over a large area, thereby producing a map of molecular flow. It does not require isolated molecules, and thus many molecules can be labeled at the same time and within the point spread function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.