ArticleBiophysical journal2009
Random walk of processive, quantum dot-labeled myosin Va molecules within the actin cortex of COS-7 cells.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Single-molecule analysis of intracellular insulin granule behavior and its application to analyzing cytoskeletal dependence and pathophysiological implications.Frontiers in physiology · 2023Article
- Mitochondria-associated myosin 19 processively transports mitochondria on actin tracks in living cells.The Journal of biological chemistry · 2022Article
- Cargo properties play a critical role in myosin Va-driven cargo transport along actin filaments.Biochemistry and biophysics reports · 2022Article
- A novel labeling strategy reveals that myosin Va and myosin Vb bind the same dendritically polarized vesicle population.Traffic (Copenhagen, Denmark) · 2020Article
- Myosin V fluorescence imaging dataset for single-molecule localization and tracking.Data in brief · 2019Article
- MAP7 regulates organelle transport by recruiting kinesin-1 to microtubules.The Journal of biological chemistry · 2019Article
- Myosin Va transport of liposomes in three-dimensional actin networks is modulated by actin filament density, position, and polarity.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Decreased intracellular granule movement and glucagon secretion in pancreatic α cells attached to superior cervical ganglion neurites.Molecular and cellular biochemistry · 2018Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Non-specific interactions govern cytosolic diffusion of nanosized objects in mammalian cells.Nature materials · 2018Article
- Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level.Nature communications · 2018Article
- Tau directs intracellular trafficking by regulating the forces exerted by kinesin and dynein teams.Traffic (Copenhagen, Denmark) · 2018Article
- Acetylated Microtubules Are Preferentially Bundled Leading to Enhanced Kinesin-1 Motility.Biophysical journal · 2017Article
- Live-cell single-molecule labeling and analysis of myosin motors with quantum dots.Molecular biology of the cell · 2017Article
- Tropomyosin isoforms bias actin track selection by vertebrate myosin Va.Molecular biology of the cell · 2016Article
- Kinetic Adaptations of Myosins for Their Diverse Cellular Functions.Traffic (Copenhagen, Denmark) · 2016Review
- Dense granule trafficking in Toxoplasma gondii requires a unique class 27 myosin and actin filaments.Molecular biology of the cell · 2016Article
- Flexural Stiffness of Myosin Va Subdomains as Measured from Tethered Particle Motion.Journal of biophysics (Hindawi Publishing Corporation : Online) · 2015Article
- The effect of surface charge on nonspecific uptake and cytotoxicity of CdSe/ZnS core/shell quantum dots.Beilstein journal of nanotechnology · 2015Article
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Abstract
Myosin Va (myoVa) is an actin-based intracellular cargo transporter. In vitro experiments have established that a single myoVa moves processively along actin tracks, but less is known about how this motor operates within cells. Here we track the movement of a quantum dot (Qdot)-labeled myoVa HMM in COS-7 cells using total internal reflectance fluorescence microscopy. This labeling approach is unique in that it allows myoVa, instead of its cargo, to be tracked. Single-particle analysis showed short periods (</=0.5 s) of ATP-sensitive linear motion. The mean velocity of these trajectories was 604 nm/s and independent of the number of myoVa molecules attached to the Qdot. With high time (16.6 ms) and spatial (15 nm) resolution imaging, Qdot-labeled myoVa moved with sequential 75 nm steps per head, at a rate of 16 s(-1), similarly to myoVa in vitro. Monte Carlo modeling suggests that the random nature of the trajectories represents processive myoVa motors undergoing a random walk through the dense and randomly oriented cortical actin network.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.