ArticleBiochemistry2009
The amino acid motif L/IIxxFE defines a novel actin-binding sequence in PDZ-RhoGEF.
Article in Biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Evolutionarily conserved short linear motifs drive actin filament binding.Nature cell biology · 2026Article
- Solo regulates the localization and activity of PDZ-RhoGEF for actin cytoskeletal remodeling in response to substrate stiffness.Molecular biology of the cell · 2024Article
- Tension-dependent RHGF-1 recruitment to stress fibers drives robust spermathecal tissue contraction.The Journal of cell biology · 2023Article
- Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions.Nature cell biology · 2020Article
- SCN9A Epileptic Encephalopathy Mutations Display a Gain-of-function Phenotype and Distinct Sensitivity to Oxcarbazepine.Neuroscience bulletin · 2020Article
- Dcf1 Affects Memory and Anxiety by Regulating NMDA and AMPA Receptors.Neurochemical research · 2019Article
- Lowe syndrome-linked endocytic adaptors direct membrane cycling kinetics with OCRL inMolecular biology of the cell · 2019Article
- Withdrawal from repeated morphine administration augments expression of the RhoA network in the nucleus accumbens to control synaptic structure.Journal of neurochemistry · 2018Article
- The dendritic spine morphogenic effects of repeated cocaine use occur through the regulation of serum response factor signaling.Molecular psychiatry · 2018Article
- Dcf1 Triggers Dendritic Spine Formation and Facilitates Memory Acquisition.Molecular neurobiology · 2018Article
- PLEKHG3 enhances polarized cell migration by activating actin filaments at the cell front.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Regulated localization is sufficient for hormonal control of regulator of G protein signaling homology Rho guanine nucleotide exchange factors (RH-RhoGEFs).The Journal of biological chemistry · 2014Article
- Identification of F-actin as the dynamic hub in a microbial-induced GTPase polarity circuit.Cell · 2012Article
- Identification of a novel actin-binding domain within the Rho guanine nucleotide exchange factor TEM4.PloS one · 2012Article
- Structure and function of heterotrimeric G protein-regulated Rho guanine nucleotide exchange factors.Molecular pharmacology · 2010Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
PDZ-RhoGEF is a member of the regulator family of G protein signaling (RGS) domain-containing RhoGEFs (RGS-RhoGEFs) that link activated heterotrimeric G protein alpha subunits of the G12 family to activation of the small GTPase RhoA. Unique among the RGS-RhoGEFs, PDZ-RhoGEF contains a short sequence that localizes the protein to the actin cytoskeleton. In this report, we demonstrate that the actin-binding domain, located between amino acids 561 and 585, directly binds to F-actin in vitro. Extensive mutagenesis identifies isoleucine 568, isoleucine 569, phenylalanine 572, and glutamic acid 573 as being necessary for binding to actin and for colocalization with the actin cytoskeleton in cells. These results define a novel actin-binding sequence in PDZ-RhoGEF with a critical amino acid motif of IIxxFE. Moreover, sequence analysis identifies a similar actin-binding motif in the N-terminus of the RhoGEF frabin, and as with PDZ-RhoGEF, mutagenesis and actin interaction experiments demonstrate an LIxxFE motif, consisting of the key amino acids leucine 23, isoleucine 24, phenylalanine 27, and glutamic acid 28. Taken together, results with PDZ-RhoGEF and frabin identify a novel actin-binding sequence. Lastly, inducible dimerization of the actin-binding region of PDZ-RhoGEF revealed a dimerization-dependent actin bundling activity in vitro. PDZ-RhoGEF exists in cells as a dimer, raising the possibility that PDZ-RhoGEF could influence actin structure in a manner independent of its ability to activate RhoA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.