ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2009
Proliferation of mouse embryonic stem cell progeny and the spontaneous contractile activity of cardiomyocytes are affected by microtopography.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Transthyretin amyloid fibrils alter primary fibroblast structure, function, and inflammatory gene expression.American journal of physiology. Heart and circulatory physiology · 2021Article
- Hang on tight: reprogramming the cell with microstructural cues.Biomedical microdevices · 2019Review
- Effective Spatial Separation of PC12 and NIH3T3 Cells by the Microgrooved Surface of Biocompatible Polymer Substrates.Langmuir : the ACS journal of surfaces and colloids · 2015Article
- Selectivity of biopolymer membranes using HepG2 cells.Regenerative biomaterials · 2015Article
- Control of stem cell fate by engineering their micro and nanoenvironment.World journal of stem cells · 2015Review
- Sustained delivery of MGF peptide from microrods attracts stem cells and reduces apoptosis of myocytes.Biomedical microdevices · 2014Article
- Cyclic strain dominates over microtopography in regulating cytoskeletal and focal adhesion remodeling of human mesenchymal stem cells.Biochemical and biophysical research communications · 2013Article
- Endothelial cells derived from embryonic stem cells respond to cues from topographical surface patterns.Journal of biological engineering · 2013Article
- Molecular mechanisms of cardiomyocyte aging.Clinical science (London, England : 1979) · 2011Review
- Differentiation of bone marrow-derived mesenchymal stem cells into hepatocyte-like cells on nanofibers and their transplantation into a carbon tetrachloride-induced liver fibrosis model.Stem cell reviews and reports · 2011Article
- Three-dimensional culture with stiff microstructures increases proliferation and slows osteogenic differentiation of human mesenchymal stem cells.Small (Weinheim an der Bergstrasse, Germany) · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The niche in which stem cells reside and differentiate is a complex physicochemical microenvironment that regulates cell function. The role played by three-dimensional physical contours was studied on cell progeny derived from mouse embryonic stem cells using microtopographies created on PDMS (poly-dimethyl-siloxane) membranes. While markers of differentiation were not affected, the proliferation of heterogeneous mouse embryonic stem cell-derived progeny was attenuated by 15 microm-, but not 5 microm-high microprojections. This reduction was reversed by Rho kinase and myosin light chain kinase inhibition, which diminishes the tension generating ability of stress fibers. Purified cardiomyocytes derived from embryonic stem cells also showed significant blunting of proliferation and increased beating rates compared with cells grown on flat substrates. Thus, proliferation of stem cell-derived progeny appears to be regulated by microtopography through tension-generation of contractility in the third-dimension. These results emphasize the importance of topographic cues in the modulation of stem cell progeny behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.