ArticleInternational journal of cancer2010
Quantitative evaluation of DNA hypermethylation in malignant and benign breast tissue and fluids.
Article in International journal of cancer, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 109 citations in OpenAlex.
- Pooled it
- Trans-resveratrol alters mammary promoter hypermethylation in women at increased risk for breast cancer.Nutrition and cancer · 2012Trial
- Nipple aspirate fluid as a proximal breast liquid biopsy platform: advancing precision risk management in breast cancer.Clinical proteomics · 2026Review
- Nutraceuticals in the Mediterranean Diet: Potential Avenues for Breast Cancer Treatment.Nutrients · 2021Review
- Resveratrol and Tumor Microenvironment: Mechanistic Basis and Therapeutic Targets.Molecules (Basel, Switzerland) · 2020Review
- Prediagnostic breast milk DNA methylation alterations in women who develop breast cancer.Human molecular genetics · 2020Article
- RASSF-1A modulates proliferation-mediated oral squamous cell carcinoma progression.Cancer cell international · 2019Article
- Methylation Profiles of BRCA1, RASSF1A and GSTP1 in Vietnamese Women with Breast CancerAsian Pacific journal of cancer prevention : APJCP · 2018Article
- Quantitative assessment of aberrantCancer management and research · 2018Review
- EBV Associated Breast Cancer Whole Methylome Analysis Reveals Viral and Developmental Enriched Pathways.Frontiers in oncology · 2018Article
- Methylation pattern analysis in prostate cancer tissue: identification of biomarkers using an MS-MLPA approach.Journal of translational medicine · 2016Article
- Blocking the PAH2 domain of Sin3A inhibits tumorigenesis and confers retinoid sensitivity in triple negative breast cancer.Oncotarget · 2016Article
- DNA promoter hypermethylation in nipple fluid: a potential tool for early breast cancer detection.Oncotarget · 2016Article
- Genomic Changes in Normal Breast Tissue in Women at Normal Risk or at High Risk for Breast Cancer.Breast cancer : basic and clinical research · 2016Review
- Resveratrol and cancer: Challenges for clinical translation.Biochimica et biophysica acta · 2015Review
- Repeated nipple fluid aspiration: compliance and feasibility results from a prospective multicenter study.PloS one · 2015Article
- Relationships among folate, alcohol consumption, gene variants in one-carbon metabolism and p16INK4a methylation and expression in healthy breast tissues.Carcinogenesis · 2015Article
- Comparison of methylation profiling in cancerous and their corresponding normal tissues from korean patients with breast cancer.Annals of laboratory medicine · 2013Article
- MethyQA: a pipeline for bisulfite-treated methylation sequencing quality assessment.BMC bioinformatics · 2013Article
- Promoter hypermethylation contributes to the frequent suppression of the CDK10 gene in human nasopharyngeal carcinomas.Cellular oncology (Dordrecht, Netherlands) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The assessment of DNA had demonstrated altered methylation in malignant compared to benign breast tissue. The purpose of our study was to (i) confirm the predictive ability of methylation assessment in breast tissue, and (ii) use the genes found to be cancer predictive in tissue to evaluate the diagnostic potential of hypermethylation assessment in nipple aspirate fluid (NAF) and mammary ductoscopic (MD) samples. Quantitative methylation specific (qMS)-PCR was conducted on three specimen sets: 44 malignant (CA) and 34 normal (NL) tissue specimens, 18 matched CA, adjacent normal (ANL) tissue and NAF specimens, and 119 MD specimens. Training and validation tissue sets were analyzed to determine the optimal group of cancer predictive genes for NAF and MD analysis. NAF and MD cytologic review were also performed. Methylation of CCND-2, p16, RAR-beta and RASSF-1a was significantly more prevalent in tumor than in normal tissue specimens. Receiver operating characteristic curve analysis demonstrated an area under the curve of 0.96. For the 18 matched CA, ANL and NAF specimens, the four predictive genes identified in cancer tissue contained increased methylation in CA vs. ANL tissue; NAF samples had higher methylation than ANL specimens. Methylation frequency was higher in MD specimens from breasts with cancer than benign samples for p16 and RASSF-1a. In summary, i) routine quantitative DNA methylation assessment in NAF and MD samples is possible, and ii) genes hypermethylated in malignant breast tissue are also altered in matched NAF and in MD samples, and may be useful to assist in early breast cancer detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.