ArticleThe Journal of biological chemistry2009
Episomal high copy number maintenance of hairpin-capped DNA bearing a replication initiation region in human cells.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 11 citations in OpenAlex.
- Article
- Targeted amplification of a sequence of interest in artificial chromosome in mammalian cells.Nucleic acids research · 2019Article
- Amplification of a transgene within a long array of replication origins favors higher gene expression in animal cells.PloS one · 2017Article
- Cloning and Characterization of a Human Genomic Sequence that Alleviates Repeat-Induced Gene Silencing.PloS one · 2016Article
- Cruciform structures are a common DNA feature important for regulating biological processes.BMC molecular biology · 2011Review
- Origin-dependent inverted-repeat amplification: a replication-based model for generating palindromic amplicons.PLoS genetics · 2011Article
- Amplification of a plasmid bearing a mammalian replication initiation region in chromosomal and extrachromosomal contexts.Nucleic acids research · 2011Article
- E. coli SbcCD and RecA control chromosomal rearrangement induced by an interrupted palindrome.Molecular cell · 2010Article
- SIRT1 stabilizes extrachromosomal gene amplification and contributes to repeat-induced gene silencing.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously found that a plasmid bearing a replication initiation region efficiently initiates gene amplification in mammalian cells and that it generates extrachromosomal double minutes and/or chromosomal homogeneously staining regions. During analysis of the underlying mechanism, we serendipitously found that hairpin-capped linear DNA was stably maintained as numerous extrachromosomal tiny episomes for more than a few months in a human cancer cell line. Generation of such episomes depended on the presence of the replication initiation region in the original plasmid. Despite extrachromosomal maintenance, episomal gene expression was epigenetically suppressed. The Southern blot analysis of the DNA of cloned cells revealed that the region around the hairpin end was diversified between the clones. Furthermore, the bisulfite-modified PCR and the sequencing analyses revealed that the palindrome sequence that derived from the original hairpin end or its end-resected structure were well preserved during clonal long term growth. From these data, we propose a model that explains the formation and maintenance of these episomes, in which replication of the hairpin-capped DNA and cruciform formation and its resolution play central roles. Our findings may be relevant for the dissection of mammalian replicator sequences.
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