ArticleThe Journal of experimental biology2009
CFTR Cl- channel functional regulation by phosphorylation of focal adhesion kinase at tyrosine 407 in osmosensitive ion transporting mitochondria rich cells of euryhaline killifish.
Article in The Journal of experimental biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- Targeting PI3Kγ anchoring enhances CFTR membrane localization and modulator efficacy via PKD1.JCI insight · 2026Article
- Beyond the mutations: spatiotemporal regulation of CFTR by cAMP and calcium signaling in epithelial physiology and cystic fibrosis.Cellular & molecular biology letters · 2025Review
- Comparative Proteomics of Salinity Stress Responses in Fish and Aquatic Invertebrates.Proteomics · 2025Review
- Ionic regulatory strategies of crabs: the transition from water to land.Frontiers in physiology · 2024Review
- Expression and Function of ABC Proteins in Fish Intestine.Frontiers in physiology · 2021Review
- Cystic Fibrosis Transmembrane Conductance Regulator: A Possible New Target for Photodynamic Therapy Enhances Wound Healing.Advances in wound care · 2019Article
- Salinity stress-induced differentially expressed miRNAs and target genes in sea cucumbers Apostichopus japonicus.Cell stress & chaperones · 2019Article
- WNK1 and p38-MAPK distribution in ionocytes and accessory cells of euryhaline teleost fish implies ionoregulatory function.Biology open · 2017Article
- Past seawater experience enhances seawater adaptability in medaka, Oryzias latipes.Zoological letters · 2016Article
- Regulation of the cystic fibrosis transmembrane conductance regulator anion channel by tyrosine phosphorylation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- MicroRNA-Sequence Profiling Reveals Novel Osmoregulatory MicroRNA Expression Patterns in Catadromous Eel Anguilla marmorata.PloS one · 2015Article
- The role of osmotic stress transcription factor 1 in fishes.Frontiers in zoology · 2014Article
- Salinity-induced regulation of the myo-inositol biosynthesis pathway in tilapia gill epithelium.The Journal of experimental biology · 2013Article
- The secret life of CFTR as a calcium-activated chloride channel.The Journal of physiology · 2013Review
- Osmoregulatory response to low salinities in the European sea bass embryos: a multi-site approach.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2013Article
- A novel role of protein tyrosine kinase2 in mediating chloride secretion in human airway epithelial cells.PloS one · 2011Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cystic fibrosis transmembrane conductance regulator (CFTR) anion channels are the regulated exit pathway in Cl(-) secretion by teleost mitochondria rich salt secreting (MR) cells of the gill and opercular epithelia of euryhaline teleosts. By confocal light immunocytochemistry, immunogold transmission electron microscopy (TEM), and co-immunoprecipitation, using regular and phospho-antibodies directed against conserved sites, we found that killifish CFTR (kfCFTR) and the tyrosine kinase focal adhesion kinase (FAK) phosphorylated at Y407 (FAK pY407) are colocalized in the apical membrane and in subjacent membrane vesicles of MR cells. We showed previously that basolateral FAK pY407, unlike other FAK phosphorylation sites, is osmosensitive and dephosphorylates during hypotonic shock of epithelial cells (Marshall et al., 2008). In the present study, we found that hypotonic shock and the alpha(2)-adrenergic agonist clonidine (neither of which affects cAMP levels) rapidly and reversibly inhibit Cl(-) secretion by isolated opercular membranes, simultaneous with dephosphorylation of FAK pY407, located in the apical membrane. FAK pY407 is rephosphorylated and Cl(-) secretion rapidly restored by hypertonic shock as well as by forskolin and isoproterenol, which operate via cAMP and protein kinase A. We conclude that hormone mediated, cAMP dependent and osmotically mediated, cAMP independent pathways converge on a mechanism to activate CFTR and Cl(-) secretion, possibly through tyrosine phosphorylation of CFTR by FAK.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.