ArticleEukaryotic cell2009
Role for chitin and chitooligomers in the capsular architecture of Cryptococcus neoformans.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 57 citations in OpenAlex.
- Article
- Article
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- Corrected and republished from: "Extracellular Vesicle Formation inInfection and immunity · 2024Article
- Preparation of Biologically Active Fractions Enriched with Glucuronoxylomannan, the Main Antigen of the Cryptococcal Capsule.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Dectin-3-targeted antifungal liposomes efficiently bind and kill diverse fungal pathogens.Molecular microbiology · 2023Article
- More complicated than it seems: The diversity of cryptococcal glucuronoxylomannan.PLoS pathogens · 2023Article
- The Cryptococcus wall: A different wall for a unique lifestyle.PLoS pathogens · 2023Article
- Extracellular Vesicle Formation in Cryptococcus deuterogattii Impacts Fungal Virulence and Requires theInfection and immunity · 2022Article
- Screening of the Pandemic Response Box Reveals an Association between Antifungal Effects of MMV1593537 and the Cell Wall ofMicrobiology spectrum · 2022Article
- Monoclonal Antibodies against Cell Wall Chitooligomers as Accessory Tools for the Control of Cryptococcosis.Antimicrobial agents and chemotherapy · 2021Article
- Erg6 affects membrane composition and virulence of the human fungal pathogen Cryptococcus neoformans.Fungal genetics and biology : FG & B · 2020Article
- Article
- The Fungal Cell Wall:Frontiers in microbiology · 2019Review
- Unraveling synthesis of the cryptococcal cell wall and capsule.Glycobiology · 2018Review
- Cryptococcal Traits Mediating Adherence to Biotic and Abiotic Surfaces.Journal of fungi (Basel, Switzerland) · 2018Review
- Lack of chitin synthase genes impacts capsular architecture and cellular physiology inCell surface (Amsterdam, Netherlands) · 2018Article
- A Predicted Mannoprotein Participates inmSphere · 2018Article
- Titan cell production inCell surface (Amsterdam, Netherlands) · 2018Article
- Peeling the onion: the outer layers of Cryptococcus neoformans.Memorias do Instituto Oswaldo Cruz · 2018Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
Molecules composed of beta-1,4-linked N-acetylglucosamine (GlcNAc) and deacetylated glucosamine units play key roles as surface constituents of the human pathogenic fungus Cryptococcus neoformans. GlcNAc is the monomeric unit of chitin and chitooligomers, which participate in the connection of capsular polysaccharides to the cryptococcal cell wall. In the present study, we evaluated the role of GlcNAc-containing structures in the assembly of the cryptococcal capsule. The in vivo expression of chitooligomers in C. neoformans varied depending on the infected tissue, as inferred from the differential reactivity of yeast forms to the wheat germ agglutinin (WGA) in infected brain and lungs of rats. Chromatographic and dynamic light-scattering analyses demonstrated that glucuronoxylomannan (GXM), the major cryptococcal capsular component, interacts with chitin and chitooligomers. When added to C. neoformans cultures, chitooligomers formed soluble complexes with GXM and interfered in capsular assembly, as manifested by aberrant capsules with defective connections with the cell wall and no reactivity with a monoclonal antibody to GXM. Cultivation of C. neoformans in the presence of an inhibitor of glucosamine 6-phosphate synthase resulted in altered expression of cell wall chitin. These cells formed capsules that were loosely connected to the cryptococcal wall and contained fibers with decreased diameters and altered monosaccharide composition. These results contribute to our understanding of the role played by chitin and chitooligosaccharides on the cryptococcal capsular structure, broadening the functional activities attributed to GlcNAc-containing structures in this biological system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.