ArticleApplied and environmental microbiology2009
Cell surface engineering of a beta-galactosidase for galactooligosaccharide synthesis.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Biochemical characterization of a novel β-galactosidase from Pedobacter sp. with strong transglycosylation activity at low lactose concentration.Folia microbiologica · 2024Article
- Biological activity of galacto-oligosaccharides: A review.Frontiers in microbiology · 2022Review
- Constitutive expression and cell-surface display of a bacterial β-mannanase in Lactobacillus plantarum.Microbial cell factories · 2019Article
- Prebiotics: Definition, Types, Sources, Mechanisms, and Clinical Applications.Foods (Basel, Switzerland) · 2019Review
- The effect of cyclodextrins on the ethanol tolerance of microorganisms suggests potential application.Journal of industrial microbiology & biotechnology · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel gene encoding transglycosylating beta-galactosidase (BGase) was cloned from Penicillium expansum F3. The sequence contained a 3,036-bp open reading frame encoding a 1,011-amino-acid protein. This gene was subsequently expressed on the cell surface of Saccharomyces cerevisiae EBY-100 by galactose induction. The BGase-anchored yeast could directly utilize lactose to produce galactooligosaccharide (GOS), as well as the by-products glucose and a small quantity of galactose. The glucose was consumed by the yeast, and the galactose was used for BGase expression, thus greatly facilitating GOS synthesis. The GOS yield reached 43.64% when the recombinant yeast was cultivated in yeast nitrogen base-Casamino Acids medium containing 100 g/liter initial lactose at 25 degrees C for 5 days. The yeast cells were harvested and recycled for the next batch of GOS synthesis. During sequential operations, both oligosaccharide synthesis and BGase expression were maintained at high levels with GOS yields of over 40%, and approximately 8 U/ml of BGase was detected in each batch.
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Registered trials
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