ArticleProceedings of the National Academy of Sciences of the United States of America1991
Characterization of a mammalian smooth muscle myosin heavy-chain gene: complete nucleotide and protein coding sequence and analysis of the 5' end of the gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 125 citations in OpenAlex.
- A cell atlas of the developing human outflow tract of the heart and its adult aortic valve derivatives.eLife · 2026Article
- The Pathological Mechanisms and Therapeutic Molecular Targets in Arteriovenous Fistula Dysfunction.International journal of molecular sciences · 2024Review
- Complex genotype-phenotype correlation of MYH11: new insights from monozygotic twins with highly variable expressivity and outcomes.BMC medical genomics · 2024Article
- MicroRNA regulation of phenotypic transformations in vascular smooth muscle: relevance to vascular remodeling.Cellular and molecular life sciences : CMLS · 2023Review
- Role of vascular smooth muscle cell clonality in atherosclerosis.Frontiers in cardiovascular medicine · 2023Review
- Circular RNA SNX29 Sponges miR-744 to Regulate Proliferation and Differentiation of Myoblasts by Activating the Wnt5a/CaMolecular therapy. Nucleic acids · 2019Article
- Loss of smooth muscle myosin heavy chain results in the bladder and stomach developing lesion during foetal development in mice.Journal of genetics · 2018Article
- LncRNA MALAT1 regulates smooth muscle cell phenotype switch via activation of autophagy.Oncotarget · 2018Article
- The expression and functional activities of smooth muscle myosin and non-muscle myosin isoforms in rat prostate.Journal of cellular and molecular medicine · 2018Article
- Vascular smooth muscle cells in cerebral aneurysm pathogenesis.Translational stroke research · 2014Review
- Effect of estrogen on molecular and functional characteristics of the rodent vaginal muscularis.The journal of sexual medicine · 2013Article
- SMB myosin heavy chain knockout enhances tonic contraction and reduces the rate of force generation in ileum and stomach antrum.American journal of physiology. Cell physiology · 2013Article
- Smooth muscle cell phenotypic switching in atherosclerosis.Cardiovascular research · 2012Review
- Generation of a human urinary bladder smooth muscle cell line.In vitro cellular & developmental biology. Animal · 2012Article
- Smooth muscle myosin expression, isoform composition, and functional activities in rat corpus cavernosum altered by the streptozotocin-induced type 1 diabetes.American journal of physiology. Endocrinology and metabolism · 2012Article
- Loop 1 dynamics in smooth muscle myosin: isoform specific differences modulate ADP release.Journal of muscle research and cell motility · 2011Article
- Smooth, slow and smart muscle motors.Journal of muscle research and cell motility · 2003Review
- Transforming growth factor-beta induction of smooth muscle cell phenotpye requires transcriptional and post-transcriptional control of serum response factor.The Journal of biological chemistry · 2002Article
- Variable surface loops and myosin activity: accessories to a motor.Journal of muscle research and cell motility · 2000Review
- Amino-acid sequence of squid myosin heavy chain.Journal of muscle research and cell motility · 1998Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The purpose of this study was to characterize the complete cDNA sequence encoding the rabbit smooth muscle myosin heavy chain (MHC) and determine the exon/intron organization at the 5' end of the corresponding gene. The full-length cDNA sequence of 6644 base pairs encoding a protein of 1972 amino acids was generated from two cDNA clones: PBRUC1 (approximately 6.3 kilobases), isolated from a rabbit uterus cDNA library, and PBRU-PCR33 (420 base pairs), produced by primer extension and PCR amplification. Compared with the chicken smooth muscle MHC sequence [Yanagisawa, M., Hamada, Y., Katsuragawa, Y., Imamura, M., Mikawa, T. & Masaki, T. (1987) J. Mol. Biol. 198, 143-157] the rabbit MHC shares about 90% amino acid identity in the S1 globular head region but shows a striking sequence divergence at the junction between the 25-kDa and 50-kDa proteolytic fragments of the functionally important S1 head domain. Genomic cloning shows that the rabbit smooth muscle MHC gene is large and has an unusual exon/intron organization at the 5' end. The first eight contiguous exons are located within a region of at least 70 kilobases of genomic DNA. Some introns span several kilobases of DNA and others at the 5' end show a high degree of intron conservation in the Mg(2+)-ATPase domain when compared with more distantly related sarcomeric MHC genes. Primer extension and S1 nuclease mapping analysis demonstrate that transcription initiates from a single site in the rabbit smooth muscle MHC gene.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.