ArticleRNA (New York, N.Y.)2009
Ntc90 is required for recruiting first step factor Yju2 but not for spliceosome activation.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Structural basis for conformational equilibrium of the catalytic spliceosome.Molecular cell · 2021Article
- Structural requirement of Ntc77 for spliceosome activation and first catalytic step.Nucleic acids research · 2014Article
- A homolog of lariat-debranching enzyme modulates turnover of branched RNA.RNA (New York, N.Y.) · 2014Article
- Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development.eLife · 2014Article
- Proteins associated with SF3a60 in T. brucei.PloS one · 2014Article
- A weak spliceosome-binding domain of Yju2 functions in the first step and bypasses Prp16 in the second step of splicing.Molecular and cellular biology · 2013Article
- Malleable ribonucleoprotein machine: protein intrinsic disorder in the Saccharomyces cerevisiae spliceosome.PeerJ · 2013Article
- Functional roles of protein splicing factors.Bioscience reports · 2012Review
- Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS.RNA (New York, N.Y.) · 2012Article
- The spliceosome-activating complex: molecular mechanisms underlying the function of a pleiotropic regulator.Frontiers in plant science · 2012Article
- Splicing factor Cwc22 is required for the function of Prp2 and for the spliceosome to escape from a futile pathway.Molecular and cellular biology · 2011Article
- Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction.Molecular and cellular biology · 2009Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Prp19-associated complex (NineTeen Complex [NTC]) is required for spliceosome activation by specifying interactions of U5 and U6 with pre-mRNA on the spliceosome after the release of U4. The NTC consists of at least eight protein components, including two tetratricopeptide repeat (TPR)-containing proteins, Ntc90 and Ntc77. Ntc90 has nine copies of the TPR with seven clustered in the carboxy-terminal half of the protein, and interacts with all identified NTC components except for Prp19 and Ntc25. It forms a stable complex with Ntc31, Ntc30, and Ntc20 in the absence of Ntc25, when other interactions between NTC components are disrupted. In this study, we used both biochemical and genetic methods to analyze the structure of Ntc90, and its function in maintaining the integrity of the NTC and in NTC-mediated spliceosome activation. Our results show that Ntc90 interacts with Ntc31, Ntc30, and other NTC components through different regions of the protein, and that its function may be regulated by Ntc31 and Ntc30. Ntc90 is not required for the association of Prp19, Ntc85, Ntc77, Ntc25, and Ntc20, or for their binding to the spliceosome. It is also not required for NTC-mediated spliceosome activation, but is required for the recruitment of Yju2, which is involved in the first catalytic reaction after the function of Prp2. Our results demonstrate a novel role of the NTC in recruiting splicing factors to the spliceosome after its activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.