ArticleAnalytical biochemistry2009
Detecting low-abundance vasoactive peptides in plasma: progress toward absolute quantitation using nano liquid chromatography-mass spectrometry.
Article in Analytical biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- Targeted LC-MS/MS platform for the comprehensive determination of peptides in the kallikrein-kinin system.Analytical and bioanalytical chemistry · 2021Article
- Counter-regulatory renin-angiotensin system in cardiovascular disease.Nature reviews. Cardiology · 2020Review
- Article
- A microanalytical capillary electrophoresis mass spectrometry assay for quantifying angiotensin peptides in the brain.Analytical and bioanalytical chemistry · 2019Article
- Diversity of Neuropeptide Cell-Cell Signaling Molecules Generated by Proteolytic Processing Revealed by Neuropeptidomics Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2018Review
- Biochemical evaluation of the renin-angiotensin system: the good, bad, and absolute?American journal of physiology. Heart and circulatory physiology · 2016Review
- Neuropeptidomics Mass Spectrometry Reveals Signaling Networks Generated by Distinct Protease Pathways in Human Systems.Journal of the American Society for Mass Spectrometry · 2015Review
- The application of Gaussian mixture models for signal quantification in MALDI-TOF mass spectrometry of peptides.PloS one · 2014Article
- Article
- Mass spectrometry for the molecular imaging of angiotensin metabolism in kidney.American journal of physiology. Endocrinology and metabolism · 2012Article
- Profiling protease activities by dynamic proteomics workflows.Proteomics · 2012Review
- A peptidomics strategy to elucidate the proteolytic pathways that inactivate peptide hormones.Biochemistry · 2011Article
- Neuropeptidomic components generated by proteomic functions in secretory vesicles for cell-cell communication.The AAPS journal · 2010Review
- Expanding the dipeptidyl peptidase 4-regulated peptidome via an optimized peptidomics platform.Journal of the American Chemical Society · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Profiling changes in the concentration of functionally related peptide hormones is critical to understanding the etiology of many diseases and therapies. We present novel data using nano liquid chromatography-mass spectrometry (LC-MS) to simultaneously measure a select group of vasoactive peptides (angiotensin, bradykinin, and related hormones) in 50-microl plasma samples, enabling repeated sampling in rodent models. By chromatographically resolving target peptides and using multiple reaction monitoring to enhance MS sensitivity, linear responses down to 10(-17) mol were achieved. Purification of plasma peptides by either methanol precipitation or off-line high-performance liquid chromatography (HPLC) fractionation enabled the detection of endogenous peptides and revealed approaches for enhancing recovery. As proof of principle, seven vasoactive peptides were profiled before, during, and after acute angiotensin-converting enzyme (ACE) inhibition in an anesthetized rat. Of note was an apparent 10-fold increase in vasodilatory bradykinin that reversed after drug infusion but relatively minor changes in angiotensin II levels. Targeted MS analysis used to profile functionally related peptides or other analytes will greatly enhance our ability to define the sequence of events regulating complex and dynamic physiological processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.